Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

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dc.contributor.author Mimura, Kallyne Kioko Oliveira [UNIFESP]
dc.contributor.author Moraes, Andreia R.
dc.contributor.author Miranda, Aline C.
dc.contributor.author Greco, Rebecca
dc.contributor.author Ansari, Tahera
dc.contributor.author Sibbons, Paul
dc.contributor.author Greco, Karin V.
dc.contributor.author Oliani, Sonia Maria [UNIFESP]
dc.date.accessioned 2020-07-31T12:47:33Z
dc.date.available 2020-07-31T12:47:33Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1038/srep35074
dc.identifier.citation Scientific Reports. London, v. 6, p. -, 2016.
dc.identifier.issn 2045-2322
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/56906
dc.description.abstract Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events en
dc.description.abstract and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent -
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.relation.ispartof Scientific Reports
dc.rights Acesso aberto
dc.title Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling en
dc.type Artigo
dc.description.affiliation Fed Univ Sao Paulo UNIFESP, Postgrad Struct & Funct Biol, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliation Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliation Sao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliation UCL, Northwick Pk Inst Med Res, Dept Surg Res, London HA1 3UJ, Middx, England
dc.description.affiliationUnifesp Post-Graduation in Structural and Functional Biology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, 04023-900, Brazil
dc.description.sponsorshipID FAPESP: 2012/21603-2
dc.description.sponsorshipID FAPESP: 2012/13041-4
dc.description.sponsorshipID FAPESP: 2014/18557-4
dc.description.sponsorshipID CNPq: 308144/2014-7
dc.description.sponsorshipID CNPq: 245859/2012-8
dc.identifier.file WOS000384923400001.pdf
dc.identifier.doi 10.1038/srep35074
dc.description.source Web of Science
dc.identifier.wos WOS:000384923400001
dc.coverage London
dc.citation.volume 6



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