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dc.contributor.authorPedrosa, Tatiana do Nascimento
dc.contributor.authorBarros, Aline Oliveira
dc.contributor.authorNogueira, Jessica Rodrigues
dc.contributor.authorFruet, Andrea Costa
dc.contributor.authorRodrigues, Isis Costa
dc.contributor.authorCalcagno, Danielle Queiroz [UNIFESP]
dc.contributor.authorSmith, Marilia de Arruda Cardoso [UNIFESP]
dc.contributor.authorde Souza, Tatiane Pereira
dc.contributor.authorde Moraes Barros, Silvia Berlanga
dc.contributor.authorde Vasconcellos, Marne Carvalho
dc.contributor.authorAraujo da Silva, Felipe Moura
dc.contributor.authorFerreira Koolen, Hector Henrique
dc.contributor.authorMaria-Engler, Silvya Stuchi
dc.contributor.authorLima, Emerson Silva
dc.date.accessioned2020-07-31T12:47:30Z
dc.date.available2020-07-31T12:47:30Z
dc.date.issued2016
dc.identifierhttp://dx.doi.org/10.1007/s00403-016-1685-0
dc.identifier.citationArchives Of Dermatological Research. New York, v. 308, n. 9, p. 643-654, 2016.
dc.identifier.issn0340-3696
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56861
dc.description.abstractSkin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as juca, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of juca trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 +/- 0.8 A mu g/mL) and LFP (IC50 = 16 +/- 0.5 A mu g/mL), was stronger than standard rutin (IC50 = 27.6 +/- 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent643-654
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofArchives Of Dermatological Research
dc.rightsAcesso restrito
dc.subjectLibidibia ferreaen
dc.subjectSkin agingen
dc.subjectMelanogenesisen
dc.subjectTyrosinaseen
dc.subjectPolyphenolsen
dc.titleAnti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extractsen
dc.typeArtigo
dc.description.affiliationFed Univ Amazonas UFAM, Fac Pharmaceut Sci, Gen Rodrigo Otavio Campos Jordao Ave 6200, BR-69077000 Manaus, AM, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, Brazil
dc.description.affiliationFed Univ Para UFPA, Res Grp Oncol, Belem, Para, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.affiliationAmazonas State Univ UEA, Dempster Mass Spectrometry Grp, Manaus, Amazonas, Brazil
dc.description.affiliationUnifespDepartment of Morphology and Genetic, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil
dc.identifier.doi10.1007/s00403-016-1685-0
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000386067500005
dc.coverageNew York
dc.citation.volume308
dc.citation.issue9


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