Protein remains stable at unusually high temperatures when solvated in aqueous mixtures of amino acid based ionic liquids

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Data
2016
Autores
Chevrot, Guillaume
Fileti, Eudes Eterno [UNIFESP]
Chaban, Vitaly V. [UNIFESP]
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Using molecular dynamics simulations, we investigated the thermal stability and real-time denaturation of a model mini-protein in four solvents: (1) water, (2) 1-ethyl-3-methylimidazolium alaninate [EMIM][ALA] (5 mol% in water), (3) methioninate [EMIM][MET] (5 mol% in water), and (4) tryptophanate [EMIM][TRP] (5 mol% in water). Upon analyzing the radius of gyration, the solvent-accessible surface area, root-mean-squared deviations, and inter-and intramolecular hydrogen bonds, we found that the mini-protein remains stable at 30-40 K higher temperatures in aqueous amino acid based ionic liquids (AAILs) than in water. This thermal stability was correlated with the thermodynamics and shear viscosity of the AAIL-containing mixtures. These results suggest that AAILs are generally favorable for protein conservation.
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Journal Of Molecular Modeling. New York, v. 22, n. 11, p. -, 2016.
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