Lambs are an important source of atypical enteropathogenic Escherichia coli in southern Brazil

Lambs are an important source of atypical enteropathogenic Escherichia coli in southern Brazil

Author Martins, Fernando H. Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Piazza, Roxane M. F. Google Scholar
Elias, Waldir P. Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Marzoa, Juan Google Scholar
Dahbi, Ghizlane Google Scholar
Mora, Azucena Google Scholar
Blanco, Miguel Google Scholar
Blanco, Jorge Google Scholar
Pelayo, Jacinta S. Google Scholar
Abstract Food-producing animals can harbor Escherichia coli strains with potential to cause diseases in humans. In this study, the presence of enteropathogenic E. coli (EPEC) was investigated in fecal samples from 130 healthy sheep (92 lambs and 38 adults) raised for meat in southern Brazil. EPEC was detected in 19.2% of the sheep examined, but only lambs were found to be positive. A total of 25 isolates was characterized and designated atypical EPEC (aEPEC) as tested negative for bfpA gene and BFP production. The presence of virulence markers linked to human disease as ehxA, paa, and IpfAO(113) was observed in 60%, 24%, and 88% of the isolates, respectively. Of the 11 serotypes identified, eight were described among human pathogenic strains, while three (O1:H8, O11:H21 and O125:H19) were not previously detected in aEPEC. Associations between intimin subtypes and phylogroups were observed, including eae-theta 2/A, eae-beta 1/B1, eae-alpha 2/B2 and eae-gamma 1/D. Although PFGE typing of 16 aEPEC isolates resulted in 14 unique pulsetypes suggesting a genetic diversity, specific clones were found to be distributed in some flocks. In conclusion, potentially pathogenic aEPEC strains are present in sheep raised for meat, particularly in lambs, which can better contribute to dissemination of these bacteria than adult animals. (C) 2016 Published by Elsevier B.V.
Keywords Lambs
Food production
Virulence factors
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Conselleria de Cultura, Education e Ordenacion Universitaria, Xunta de Galicia
European Regional Development Fund (ERDF)
Ministerio de Economia y Competitividad, Gobierno de Espana
Grant number ERDF: CN2012/303
Ministerio de Economia y Competitividad, Gobierno de Espana: AGL2013-47852-R
Date 2016
Published in Veterinary Microbiology. Amsterdam, v. 196, p. 72-77, 2016.
ISSN 0378-1135 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 72-77
Access rights Closed access
Type Article
Web of Science ID WOS:000390742600013

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