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dc.contributor.authordas Chagas e Silva de Carvalho, Luis Felipe
dc.contributor.authorPereira, Thiago Martini [UNIFESP]
dc.contributor.authorMagrini, Taciana Depra
dc.contributor.authorRodrigues Cavalcante, Ana Sueli
dc.contributor.authorMartinho, Herculano da Silva
dc.contributor.authorAlmeida, Janete Dias
dc.identifier.citationPhotodiagnosis And Photodynamic Therapy. Amsterdam, v. 16, p. 27-34, 2016.
dc.description.abstractActinic cheilitis (AC) is considered a potentially malignant disorder of the lip. Biomolecular markers study is important to understand malignant transformation into squamous cell carcinoma. Fourier transform infra red (FT-IR) spectroscopy was used to analyze AC in this study. Objectives: The aim of the study was to evaluate if FT-IR spectral regions of nucleic acids and collagen can help in early diagnosis of malignant transformation. Methods: Tissues biopsies of 14 patients diagnosed with AC and 14 normal tissues were obtained. FTIR spectra were measured at five different points resulting in 70 spectra of each. Analysis of Principal components analysis (PCA) and linear discrimination analysis (LDA) model were also used. In order to verify the statistical difference in the spectra, Mann-Whitney U test was performed in each variable (wavenumber) with p-value <0.05. Results: After the Mann-Whitney U test the vibrational modes of CO (Collagen 1), PO2 (Nucleic Acids) and C=0 asymmetric (Triglycerides/Lipids) were observed as a possible spectral biomarker. These bands were chosen because they represent the vibrational modes related to collagen and DNA, which are supposed to be changed in AC samples. Based on the PCA-LDA results, the predictive model corresponding to the area under the curve was 0.91 for the fingerprint region and 0.83 for the high wavenumber region, showing the greater accuracy of the test. Conclusions: FT-IR changes in collagen and nucleic acids could be used as molecular biomarkers for malignant transformation. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherElsevier Science Bv
dc.relation.ispartofPhotodiagnosis And Photodynamic Therapy
dc.rightsAcesso restrito
dc.subjectActinic cheilitisen
dc.subjectOptical biopsyen
dc.subjectFT-IR spectroscopyen
dc.subjectOral canceren
dc.titleOptical diagnosis of actinic cheilitis by infrared spectroscopyen
dc.description.affiliationUniv Vale do Paraiba, Lab Biomed Vibrat Spect, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biosci & Oral Diag, Inst Sci & Technol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed ABC, Ctr Ciencias Nat & Humanas, Sao Paulo, Brazil
dc.description.affiliationUnifespInstituto de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos campos, São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2012/50935-8
dc.description.sponsorshipIDFAPESP: 2011/06615-8
dc.description.sourceWeb of Science

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