Optical diagnosis of actinic cheilitis by infrared spectroscopy

Optical diagnosis of actinic cheilitis by infrared spectroscopy

Author das Chagas e Silva de Carvalho, Luis Felipe Google Scholar
Pereira, Thiago Martini Autor UNIFESP Google Scholar
Magrini, Taciana Depra Google Scholar
Rodrigues Cavalcante, Ana Sueli Google Scholar
Martinho, Herculano da Silva Google Scholar
Almeida, Janete Dias Google Scholar
Abstract Actinic cheilitis (AC) is considered a potentially malignant disorder of the lip. Biomolecular markers study is important to understand malignant transformation into squamous cell carcinoma. Fourier transform infra red (FT-IR) spectroscopy was used to analyze AC in this study. Objectives: The aim of the study was to evaluate if FT-IR spectral regions of nucleic acids and collagen can help in early diagnosis of malignant transformation. Methods: Tissues biopsies of 14 patients diagnosed with AC and 14 normal tissues were obtained. FTIR spectra were measured at five different points resulting in 70 spectra of each. Analysis of Principal components analysis (PCA) and linear discrimination analysis (LDA) model were also used. In order to verify the statistical difference in the spectra, Mann-Whitney U test was performed in each variable (wavenumber) with p-value <0.05. Results: After the Mann-Whitney U test the vibrational modes of CO (Collagen 1), PO2 (Nucleic Acids) and C=0 asymmetric (Triglycerides/Lipids) were observed as a possible spectral biomarker. These bands were chosen because they represent the vibrational modes related to collagen and DNA, which are supposed to be changed in AC samples. Based on the PCA-LDA results, the predictive model corresponding to the area under the curve was 0.91 for the fingerprint region and 0.83 for the high wavenumber region, showing the greater accuracy of the test. Conclusions: FT-IR changes in collagen and nucleic acids could be used as molecular biomarkers for malignant transformation. (C) 2016 Elsevier B.V. All rights reserved.
Keywords Actinic cheilitis
Optical biopsy
FT-IR spectroscopy
Oral cancer
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2012/50935-8
FAPESP: 2011/06615-8
Date 2016
Published in Photodiagnosis And Photodynamic Therapy. Amsterdam, v. 16, p. 27-34, 2016.
ISSN 1572-1000 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 27-34
Origin http://dx.doi.org/10.1016/j.pdpdt.2016.07.013
Access rights Closed access
Type Article
Web of Science ID WOS:000389731900007
URI https://repositorio.unifesp.br/handle/11600/56644

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