Absence of mycobacterial DNA in peripheral blood and artery specimens in patients with Takayasu arteritis

Absence of mycobacterial DNA in peripheral blood and artery specimens in patients with Takayasu arteritis

Author Carvalho, Evanir S. Autor UNIFESP Google Scholar
Souza, Alexandre Wagner Silva de Autor UNIFESP Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Levy-Neto, Mauricio Google Scholar
de Oliveira, Rosangela Siqueira Google Scholar
Drake, Wonder Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Saldiva, Paulo Hilário Nascimento Google Scholar
Gutierrez, Paulo Sampaio Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Abstract The objective of this study was to demonstrate the presence of mycobacterial nucleic acid sequences in peripheral blood and arteries from patients with Takayasu arteritis (TA). Polymerase chain reaction was performed to detect mycobacterial DNA from three different nucleic acid sequences including the insertion sequence (IS) 6110, the 65-kDa heat shock protein gene (HSP65), and the 16S ribosomal RNA (rRNA) gene in peripheral blood from 32 TA patients and in arterial specimens from 10 TA patients. Twenty-eight HIV-negative patients with pulmonary tuberculosis prior to therapy were tested for IS6110 in peripheral blood as positive controls, and 24 blood donors were evaluated as healthy controls (HC). All TA patients were negative for the insertion sequence IS6110 and for HSP65 and 16S rRNA genes in blood samples and in arterial specimens. IS6110 sequence was found in peripheral blood from 22 (78.5 %) patients with pulmonary tuberculosis but not in HC. In conclusion, the strategy of mycobacterial-specific nucleic acid amplification in the peripheral blood and arterial specimens of TA patients was unable to lend support to the association between TA and tuberculosis long suggested in the literature.
Keywords DNA
Mycobacterium tuberculosis
Polymerase chain reaction
Takayasu arteritis
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 98/13412-3
Date 2017
Published in Clinical Rheumatology. London, v. 36, n. 1, p. 205-208, 2017.
ISSN 0770-3198 (Sherpa/Romeo, impact factor)
Publisher Springer London Ltd
Extent 205-208
Origin http://dx.doi.org/10.1007/s10067-016-3400-0
Access rights Closed access
Type Article
Web of Science ID WOS:000392309000026
URI https://repositorio.unifesp.br/handle/11600/56481

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