Phosphosite-specific regulation of the oxidative-stress response of Paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis

Phosphosite-specific regulation of the oxidative-stress response of Paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis

Author Chaves, Alison Felipe Alencar Autor UNIFESP Google Scholar
Castilho, Daniele Goncalves Autor UNIFESP Google Scholar
Navarro, Marina Valente Autor UNIFESP Google Scholar
Oliveira, Ana K. Google Scholar
Serrano, Solange M. T. Google Scholar
Tashima, Alexandre Keiji Autor UNIFESP Google Scholar
Batista, Wagner Luiz Autor UNIFESP Google Scholar
Abstract Paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in Latin America. This fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. Therefore, the survival of P. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. For several years, reactive oxygen species (ROS) were only associated with pathological processes. Currently, a plethora of roles for ROS in cell signaling have emerged. We have previously reported that low ROS concentrations cause cell proliferation in the human pathogenic fungus P. brasiliensis. In the present report, we investigated the influence of phosphorylation events in that process. Using a mass spectrometry-based approach, we mapped 440 phosphorylation sites in 230 P. brasiliensis proteins and showed that phosphorylation at different sites determines fungal responses to oxidative stress, which are regulated by phosphatases and kinases activities. Furthermore, we present additional evidence for a functional two-component signal transduction system in P. brasiliensis. These findings will help us to understand the phosphorylation events involved in the oxidative stress response. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords Oxidative stress
Post-translational modification
Paracoccidioides brasiliensis
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)]
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 471015/2011-3
FAPESP: 2011/14392-2
FAPESP: 2014/13961-1
FAPESP: 2012/19321-9
FAPESP: CeTICS-CEPID 2013/07467-1
FAPESP: 2012/17530-0
Date 2017
Published in Microbes And Infection. Amsterdam, v. 19, n. 1, p. 34-46, 2017.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 34-46
Access rights Closed access
Type Article
Web of Science ID WOS:000392677400004

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account