Zoanthid mucus as new source of useful biologically active proteins

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dc.contributor.author Guarnieri, Miriam Camargo [UNIFESP]
dc.contributor.author Modesto, Jeanne Claine de Albuquerque
dc.contributor.author Perez, Carlos Daniel
dc.contributor.author Ottaiano, Tatiana Fontes [UNIFESP]
dc.contributor.author Ferreira, Rodrigo da Silva [UNIFESP]
dc.contributor.author Batista, Fabricio Pereira [UNIFESP]
dc.contributor.author Brito, Marlon Vilela de [UNIFESP]
dc.contributor.author Campos, Ikaro Henrique Mendes Pinto
dc.contributor.author Oliva, Maria Luiza Vilela [UNIFESP]
dc.date.accessioned 2020-07-20T16:31:22Z
dc.date.available 2020-07-20T16:31:22Z
dc.date.issued 2018
dc.identifier http://dx.doi.org/10.1016/j.toxicon.2018.01.012
dc.identifier.citation Toxicon. Oxford, v. 143, p. 96-107, 2018.
dc.identifier.issn 0041-0101
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/55921
dc.description.abstract Palythoa caribaeorum is a very common colonial zoanthid in the coastal reefs of Brazil. It is known for its massive production of mucus, which is traditionally used in folk medicine by fishermen in northeastern Brazil. This study identified biologically active compounds in P caribaerum mucus. Crude mucus was collected during low tides by the manual scraping of colonies en
dc.description.abstract samples were maintained in an ice bath, homogenized, and centrifuged at 16,000 g for 1 hat 4 degrees C en
dc.description.abstract the supernatant (mucus) was kept at -80 degrees C until use. The enzymatic (proteolytic and phospholipase A(2)), inhibitory (metallo, cysteine and serine proteases), and hemagglutinating (human erythrocyte) activities were determined. The results showed high levels of cysteine and metallo proteases, intermediate levels of phosholipase A(2), low levels of trypsin, and no elastase and chymotrypsin like activities. The mucus showed potent inhibitory activity on snake venom metalloproteases and cysteine proteinase papain. In addition, it showed agglutinating activity towards O+, B+, and A(+) erythrocyte types. The hemostatic results showed that the mucus prolongs the aPTT and PT, and strongly inhibited platelet aggregation induced by arachidonic acid, collagen, epinephrine, ADP, and thrombin. The antimicrobial activity was tested on 15 strains of bacteria and fungi through the radial diffusion assay in agar, and no activity was observed. Compounds in P. caribaeorum mucus were analyzed for the first time in this study, and our results show potential pharmacological activities in these compounds, which are relevant for use in physiopathological investigations. However, the demonstration of these activities indicates caution in the use of crude mucus in folk medicine. Furthermore, the present or absent activities identified in this mucus suggest that the studied P caribaeorum colonies were in thermal stress conditions at the time of sample collection en
dc.description.abstract these conditions may precede the bleaching process in zoanthids. Hence, the use of mucus as an indicator of this process should be evaluated in the future. (C) 2018 Published by Elsevier Ltd. en
dc.description.sponsorship FACEPE
dc.description.sponsorship FAPESP
dc.description.sponsorship CNPq
dc.description.sponsorship CAPES
dc.format.extent 96-107
dc.language.iso eng
dc.publisher Pergamon-Elsevier Science Ltd
dc.relation.ispartof Toxicon
dc.rights Acesso restrito
dc.subject Proteases en
dc.subject Inhibitors en
dc.subject Hemostasis en
dc.subject Cnidaria en
dc.subject Thermal stress en
dc.subject Palythoa en
dc.subject Metalloprotease en
dc.title Zoanthid mucus as new source of useful biologically active proteins en
dc.type Artigo
dc.description.affiliation Univ Fed Pernambuco, Dept Zool, Av Prof Moraes Rego 1235,Cidade Univ, BR-50670901 Recife, PE, Brazil
dc.description.affiliation Univ Fed Pernambuco, Vitoria Acad Ctr, Rua Alto Reservatorio S-N, BR-55608680 Vitoria De Santo Antao, PE, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Biochem Dept, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Biochem Dept, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sponsorshipID CAPES: AUXPE 140/2015
dc.identifier.doi 10.1016/j.toxicon.2018.01.012
dc.description.source Web of Science
dc.identifier.wos WOS:000426223300012
dc.coverage Oxford
dc.citation.volume v. 143



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