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dc.contributor.authorGrabulosa, Caren Cristina [UNIFESP]
dc.contributor.authorManfredi, Silvia Regina [UNIFESP]
dc.contributor.authorCanziani, Maria Eugênia Fernandes [UNIFESP]
dc.contributor.authorQuinto, Beata Marie Redublo [UNIFESP]
dc.contributor.authorBarbosa, Rodrigo B.
dc.contributor.authorRebello, Jacqueline F.
dc.contributor.authorBatista, Marcelo Costa [UNIFESP]
dc.contributor.authorCendoroglo, Miguel [UNIFESP]
dc.contributor.authorDalboni, Maria Aparecida [UNIFESP]
dc.date.accessioned2020-07-20T16:31:00Z
dc.date.available2020-07-20T16:31:00Z
dc.date.issued2018
dc.identifierhttp://dx.doi.org/10.1016/j.yexcr.2018.02.022
dc.identifier.citationExperimental Cell Research. San Diego, v. 365, n. 2, p. 157-162, 2018.
dc.identifier.issn0014-4827
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55634
dc.description.abstractTLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 expression in neutrophils and monocytes from HD and CKD patients. Blood samples were drawn from 47 CKD and 43 HD patients and 71 age and gender-matched healthy volunteers (CONT). TLR4 was analyzed using flow cytometry. Cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 were analyzed via ELISA.TLR4 expression in neutrophils was higher in HD patients than in stage 3 and 4 CKD patients. In these cells, we observed a positive correlation between TLR4 and cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 levels. In monocytes, TLR4 expression was significantly higher in CKD 3 and 4 groups than in the control and HD groups and positively and negatively correlated with IL-6 and MCP-1 and cathelicidin, respectively. TNF-alpha, IL-6 and MCP-1 serum levels were higher in HD and CKD patients than in control. Cathelicidin and IL-10 levels were only higher in HD patients. IL-6 serum levels were positively correlated with all cytokines, and cathelicidin was negatively correlated with MCP-1 (r = - 0.35en
dc.description.abstractp < 0.01) and positively correlated with IL-10 (r = 0.37en
dc.description.abstractp = 0.001). These results suggest that a uremic environment induces high TLR4, cathelicidin and cytokine expression and may increase inflammation. Thus, future studies should be conducted to evaluate whether TLR4 and cathelicidin should be targets for anti-inflammatory therapeutic strategies.en
dc.description.sponsorshipFAPESP
dc.format.extent157-162
dc.language.isoeng
dc.publisherElsevier Inc
dc.relation.ispartofExperimental Cell Research
dc.rightsAcesso restrito
dc.subjectToll like receptorsen
dc.subjectToll like receptorsen
dc.subjectInflammationen
dc.subjectInflammationen
dc.subjectChronic kidney diseaseen
dc.subjectChronic kidney diseaseen
dc.subjectImmunologyen
dc.subjectImmunologyen
dc.subjectCathelicidinen
dc.subjectCathelicidinen
dc.titleChronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytesen
dc.typeArtigo
dc.description.affiliationUniv Fed Sao Paulo, Nephrol Div, Sao Paulo, Brazil
dc.description.affiliationUniv Nove Julho, Sao Paulo, Brazil
dc.description.affiliationTufts Univ New England Med Ctr, Boston, MA USA
dc.description.affiliationHosp Israelita Albert Einstein, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Nephrol Div, Sao Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2010/52180-0
dc.description.sponsorshipIDFAPESP: 2011/51496-0
dc.identifier.doi10.1016/j.yexcr.2018.02.022
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000429630000001
dc.coverageSan Diego
dc.citation.volumev. 365
dc.citation.issue2


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