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dc.contributor.authorMenezes, Maira Maria
dc.contributor.authorNobre, Leonardo Thiago Duarte Barreto [UNIFESP]
dc.contributor.authorRossi, Gustavo Rodrigues
dc.contributor.authorAlmeida-Lima, Jailma
dc.contributor.authorMelo-Silveira, Raniere Fagundes
dc.contributor.authorFranco, Celia Regina Cavichiolo [UNIFESP]
dc.contributor.authorTrindade, Edvaldo da Silva [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorRocha, Hugo Alexandre de Oliveira [UNIFESP]
dc.identifier.citationInternational Journal Of Biological Macromolecules. Amsterdam, v. 111, p. 1067-1075, 2018.
dc.description.abstractA low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schroederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012-0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentration dependent manner. In addition, fucan B (0.01-0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B. (C) 2018 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq (National Council for Scientific and Technological Advancement)
dc.description.sponsorshipCoordenacao de Aperfeicoamento Pessoal de Nivel Superior CAPES (Higher Level Personal Development Coordination)
dc.description.sponsorshipPrograma Nacional de Cooperacao Academica (PROCAD)
dc.description.sponsorshipMinisterio de Ciencia, Tecnologia, Informacao e Comercio (MCTIC - the Science and Technology Ministry in Brazil)
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Journal Of Biological Macromolecules
dc.rightsAcesso restrito
dc.subjectBrown seaweed, endothelial cellsen
dc.titleA low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitroen
dc.description.affiliationUniv Fed Rio Grande Norte UFRN, Ctr Biociencias, Dept Bioquim, Lab Biotecnol Polimeros Nat BIOPOL, Natal, RN, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Disciplina Biol Mol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Parana, Dept Biol Celular, Curitiba, PR, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Disciplina Biol Mol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.citation.volumev. 111

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