A low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitro

A low-molecular-weight galactofucan from the seaweed, Spatoglossum schroederi, binds fibronectin and inhibits capillary-like tube formation in vitro

Author Menezes, Maira Maria Google Scholar
Nobre, Leonardo Thiago Duarte Barreto Autor UNIFESP Google Scholar
Rossi, Gustavo Rodrigues Google Scholar
Almeida-Lima, Jailma Google Scholar
Melo-Silveira, Raniere Fagundes Google Scholar
Franco, Celia Regina Cavichiolo Autor UNIFESP Google Scholar
Trindade, Edvaldo da Silva Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Rocha, Hugo Alexandre de Oliveira Autor UNIFESP Google Scholar
Abstract A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schroederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012-0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentration dependent manner. In addition, fucan B (0.01-0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B. (C) 2018 Elsevier B.V. All rights reserved.
Keywords Fucoidan
Brown seaweed, endothelial cells
Anti-angiogenic
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq (National Council for Scientific and Technological Advancement)
Coordenacao de Aperfeicoamento Pessoal de Nivel Superior CAPES (Higher Level Personal Development Coordination)
Programa Nacional de Cooperacao Academica (PROCAD)
Ministerio de Ciencia, Tecnologia, Informacao e Comercio (MCTIC - the Science and Technology Ministry in Brazil)
CAPES
Date 2018
Published in International Journal Of Biological Macromolecules. Amsterdam, v. 111, p. 1067-1075, 2018.
ISSN 0141-8130 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 1067-1075
Origin http://dx.doi.org/10.1016/j.ijbiomac.2018.01.119
Access rights Closed access
Type Article
Web of Science ID WOS:000429391000120
URI https://repositorio.unifesp.br/handle/11600/55608

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