Retinal Toxicity of Acai Fruit (Euterpe Oleracea) Dye Concentrations in Rabbits: Basic Principles of a New Dye for Chromovitrectomy in Humans

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Data
2017
Autores
Caiado, Rafael R. [UNIFESP]
Peris, Cristiane S. [UNIFESP]
Souza Lima-Filho, Acacio Alves [UNIFESP]
Palma Urushima, Joao Guilherme [UNIFESP]
Novais, Eduardo [UNIFESP]
Badaro, Emmerson [UNIFESP]
Maia, Andre [UNIFESP]
Sinigaglia-Coimbra, Rita [UNIFESP]
Watanabe, Sung Eun S.
Rodrigues, Eduardo B. [UNIFESP]
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Purpose: Evaluate toxicity of acai fruit (Euterpe oleracea) dye concentrations in a rabbit model.Methods: Rabbits were injected intravitreously with 10%, 25%, and 35% acai dye concentrations. Control eyes received balanced salt solution (BSS). Electroretinogram (ERG), fundus imaging, fluorescein angiography (FA), optical coherence tomography (OCT), and light and transmission electron microscopy (LM/TEM) were performed.Results: Fundus imaging showed increased vitreous opacity with increased dye concentrations. FA and OCT showed normality with all concentrations. Comparisons between BSS and dye concentrations were analyzed using Kruskal-Wallis and Mood's median test (p < 0.05). At 24 h, ERGs showed reduced amplitudes from baseline in all eyes. Median b-wave amplitudes nonsignificantly decreased and latency increased with 10% and 25%
findings were significant (p < 0.05) for 35%. LM and TEM showed no abnormalities for 10% and 25%. With 35%, TEM showed ganglion cell edema at 24 h that resolved after 7 days. Vacuolization, multilamellar bodies, and nerve bundle damage occurred at 24 h/7 days in the inner nuclear layer. Mitochondrial cristae disruption occurred in the inner photoreceptor segment at 24 h that decreased by 7 days.Conclusion: Ten and twenty-five percent concentrations were safe and may improve identification of the posterior hyaloid and internal limiting membrane during chromovitrectomy in humans.
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Current Eye Research. Philadelphia, v. 42, n. 8, p. 1185-1193, 2017.
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