Effects of different fluences of low-level laser therapy in an experimental model of spinal cord injury in rats

Effects of different fluences of low-level laser therapy in an experimental model of spinal cord injury in rats

Author Veronez, Suellen de Oliveira Autor UNIFESP Google Scholar
Assis, Livia Autor UNIFESP Google Scholar
Del Campo, Paula Autor UNIFESP Google Scholar
Oliveira, Flavia de Autor UNIFESP Google Scholar
Castro, Glaucia Monteiro de Autor UNIFESP Google Scholar
Renno, Ana Claudia Muniz Autor UNIFESP Google Scholar
Medalha, Carla Christina Autor UNIFESP Google Scholar
Abstract The aim of this study was to evaluate the in vivo response of different fluences of low-level laser therapy (LLLT) on the area of the injury, inflammatory markers, and functional recovery using an experimental model of traumatic spinal cord injury (SCI). Thirty two rats were randomly divided into four experimental groups: control group (CG), laser-treated group 500 J/cm(2) (L-500), laser-treated group 750 J/cm(2) (L-750), and laser-treated group 1000 J/cm(2) (L-1000). SCI was performed by an impactor equipment (between the ninth and tenth thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for seven consecutive sessions, using an 808-nm laser, at the respective fluence of each experimental groups. Motor function and tactile sensitivity were performed on days 1 and 7 post-surgery. Animals were euthanized on the eighth day after injury, and the samples were retrieved for histological and immunohistochemistry analyses. Functional evaluation and tactile sensitivity were improved after LLLT, at the higher fluence. Additionally, LLLT, at 750 and 1000 J/cm(2), reduces the lesion volume and modulates the inflammatory process with decrease of CD-68 protein expression. These results suggest that LLLT at higher doses was effective in promoting functional recovery and modulating inflammatory process in the spinal cord of rats after SCI.
Keywords Low-level laser therapy
Spinal cord injury
Neuronal plasticity
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Date 2017
Published in Lasers In Medical Science. London, v. 32, n. 2, p. 343-349, 2017.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer London Ltd
Extent 343-349
Origin https://dx.doi.org/10.1007/s10103-016-2120-7
Access rights Closed access
Type Article
Web of Science ID WOS:000394154600011
URI https://repositorio.unifesp.br/handle/11600/55190

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account