Effects of in vitro, acute and chronic treatment with fluoxetine on the sympathetic neurotransmission of rat vas deferens

Effects of in vitro, acute and chronic treatment with fluoxetine on the sympathetic neurotransmission of rat vas deferens

Author Pedroso, Shaista Poppe Autor UNIFESP Google Scholar
de Souza, Bruno Palmieri Autor UNIFESP Google Scholar
Jurkiewicz, Aron Autor UNIFESP Google Scholar
Juriewicz, Neide H. Autor UNIFESP Google Scholar
da Silva Junior, Edilson Dantas Autor UNIFESP Google Scholar
Abstract It is described that fluoxetine treatment is able to induce ejaculatory disorders. However, the exact mechanism is still not fully understood. Therefore, this study was carried out to further evaluate the anti-ejaculatory effects of fluoxetine, using different approaches (in vitro or in vivo treatments), on the sympathetic neurotransmission of the rat vas deferens. Vas deferens from male Wistar rats were used to check the in vitro effects of fluoxetine 10(-6) M, 3.10(-6) M or 10(-5) M. Animals were also acutely (20 mg/kg, i.p. 4 h or 24 h) or chronically (10 mg/kg, i.p., 30 days) treated with fluoxetine or drug-free vehicle. The vas deferens from non -treated and treated animals were isolated and mounted in an isolated organ bath for the study of the contractions induced by adrenergic agonists, tyramine, 5-HT, Ca2+ or electrical field stimulation. In vitro or acute treatment with fluoxetine decreased the contraction induced by agonists, Ca2+ or electrical field stimulation. The chronic treatment with fluoxetine decreased the contractions induced agonists, tyramine or Ca2+, but did not modify the contractions induced by electrical field stimulation. We have shown that in vitro or in vivo fluoxetine treatment is able to alter the sympathetic neurotransmission of the rat vas deferens which could be related to alterations in the calcium signalling. (C) 2016 Elsevier B.V. All rights reserved.
Keywords Fluoxetine
Vas deferens
Sympathetic neurotransmission
In vitro or in vivo treatments
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor CAPES
FAPESP
CNPq
Date 2017
Published in Autonomic Neuroscience-Basic & Clinical. Amsterdam, v. 203, p. 17-24, 2017.
ISSN 1566-0702 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 17-24
Origin http://dx.doi.org/10.1016/j.autneu.2016.10.006
Access rights Closed access
Type Article
Web of Science ID WOS:000397358100003
URI https://repositorio.unifesp.br/handle/11600/55037

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