Intrauterine growth restriction increases circulating mitochondrial DNA and Toll-like receptor 9 expression in adult offspring: could aerobic training counteract these adaptations?

Intrauterine growth restriction increases circulating mitochondrial DNA and Toll-like receptor 9 expression in adult offspring: could aerobic training counteract these adaptations?

Author Oliveira, V. Autor UNIFESP Google Scholar
Silva Junior, S. D. Google Scholar
de Carvalho, M. H. C. Google Scholar
Akamine, E. H. Google Scholar
Michelini, L. C. Google Scholar
Franco, M. C. Autor UNIFESP Google Scholar
Abstract It has been demonstrated that intrauterine growth restriction (IUGR) can program increase cardiometabolic risk. There are also evidences of the correlation between IUGR with low-grade inflammation and, thus can contribute to development of several cardiometabolic comorbidities. Therefore, we investigated the influence of IUGR on circulating mitochondrial DNA (mtDNA)/Toll-like receptor 9 (TLR9) and TNF-alpha expression in adult offspring. Considering that the aerobic training has anti-inflammatory actions, we also investigated whether aerobic training would improve these inflammatory factors. Pregnant Wistar rats received ad libitum or 50% of ad libitum diet throughout gestation. At 8 weeks of age, male offspring from both groups were randomly assigned to control, trained control, restricted and trained restricted. Aerobic training protocol was performed on a treadmill and after that, we evaluated circulating mtDNA, cardiac protein expression of TLR9, plasma and cardiac TNF-levels, and left ventricle (LV) mass. We found that IUGR promoted an increase in the circulating mtDNA, TLR9 expression and plasma TNF-alpha levels. Further, our results revealed that aerobic training can restore mtDNA/TLR9 content and plasma levels of TNF-alpha among restricted rats. The cardiac TNF-alpha content and LV mass were not influenced either by IUGR or aerobic training. In conclusion, IUGR can program mtDNA/TLR9 content, which may lead to high levels of TNF-alpha. However, aerobic training was able to normalize these alterations. These findings evidenced that the association of IUGR and aerobic training seems to exert an important interaction effect regarding pro-inflammatory condition and, aerobic training may be used as a strategy to reduce deleterious adaptations in IUGR offspring.
Keywords aerobic training
fetal programming
heart
intrauterine growth restriction
xmlui.dri2xhtml.METS-1.0.item-coverage Cambridge
Language English
Sponsor FAPESP ( Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil)
Grant number FAPESP: 2013/03139-0
FAPESP: 2013/00311-6
Date 2017
Published in Journal Of Developmental Origins Of Health And Disease. Cambridge, v. 8, n. 2, p. 236-243, 2017.
ISSN 2040-1744 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 236-243
Origin http://dx.doi.org/10.1017/S2040174416000714
Access rights Closed access
Type Article
Web of Science ID WOS:000395508600012
URI https://repositorio.unifesp.br/handle/11600/54884

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