The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

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Date
1997-11-01Author
Santos, Marcia Regina Machado [UNIFESP]
Cano, Maria I [UNIFESP]
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J
Ramirez, José Luis
Brandão, Adeílton
Degrave, Wim M
Franco da Silveira, José [UNIFESP]
Type
ArtigoISSN
0074-0276Is part of
Memórias do Instituto Oswaldo CruzDOI
10.1590/S0074-02761997000600018Metadata
Show full item recordAbstract
By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes
Citation
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.Keywords
Trypanosoma cruziclone CL
Brener
genome project
molecular karyotype
chromosome mapping
genetic map
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