Inhibitory effect of a lipopeptide biosurfactant produced by Bacillus subtilis on planktonic and sessile cells of Trichosporon spp.

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Cordeiro, Rossana de Aguiar
Caracas Cedro, Ewerton Weslley
Colares Andrade, Ana Raquel
Serpa, Rosana
de Jesus Evangelista, Antonio Jose
de Oliveira, Jonathas Sales
Pereira, Vandbergue Santos
Alencar, Lucas Pereira
Leite Mendes, Patricia Bruna
Soares Farias, Barbara Cibelle
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The present study aimed to investigate the inhibitory effect of a bacterial biosurfactant (TIM96) on clinical strains of Trichosporon. Additionally, the effect of TIM96 on the ergosterol content, cell membrane integrity, and the hydrophobicity of planktonic cells was assessed. The inhibitory activity of TIM96 against Trichosporon biofilms was evaluated by analyzing metabolic activity, biomass and morphology. MIC values ranged from 78.125 to 312.5g ml(-1) for TIM96
time-kill curves revealed that the decline in the number of fungal cells started after incubation for 6h with TIM96 at both MIC and 2xMIC. The biosurfactant reduced the cellular ergosterol content and altered the membrane permeability and the surface hydrophobicity of planktonic cells. Incubation at 10xMIC TIM96 reduced cell adhesion by up to 96.89%, thus interfering with biofilm formation. This concentration also caused up to a 99.2% reduction in the metabolic activity of mature biofilms. The results indicate potential perspectives for the development of new antifungal strategies.
Biofouling. Abingdon, v. 34, n. 3, p. 309-319, 2018.