Nandrolone combined with strenuous resistance training reduces vascular nitric oxide bioavailability and impairs endothelium-dependent vasodilation

Nandrolone combined with strenuous resistance training reduces vascular nitric oxide bioavailability and impairs endothelium-dependent vasodilation

Author Guzzoni, Vinicius Google Scholar
Cunha, Tatiana Sousa Autor UNIFESP Google Scholar
das Neves, Vander Jose Google Scholar
Briet, Larissa Google Scholar
Costa, Rafaela Google Scholar
Costa Sampaio Moura, Maria Jose Google Scholar
Oliveira, Vanessa Autor UNIFESP Google Scholar
Pinho Franco, Maria do Carmo Autor UNIFESP Google Scholar
Novaes, Pedro Duarte Google Scholar
Marcondes, Fernanda Klein Google Scholar
Abstract Anabolic Androgenic Steroids (AASs) misuse has increased among adolescents and recreational athletes due to their potential effects on muscle hypertrophy. On the other hand, AAS might induce alterations on cardiovascular system, although some controversies regarding AAS on vascular properties remain unknown. To address this question, we aimed to investigate the effects of high doses of nandrolone combined with strenuous resistance training (RT) on function and structure of thoracic aorta. Rats were randomized into four groups: non-trained vehicle (NTV), trained vehicle (TV), non-trained nandrolone (NTN), and trained nandrolone (TN), and submitted to 6 weeks of treatment with nandrolone (5 mg/kg, twice a week) and/or resistance training. In vitro response of thoracic aorta to acetylcholine (ACh) was analyzed. Vascular nitric oxide (NO) and reactive oxygen species (ROS) synthesis were evaluated using 4,5-diaminofluorescein diacetate (DAF-2) and hydroethidine fluorescent techniques, respectively. Thoracic aorta was processed for microscopy analyses and tunica media thickness was measured. ACh-mediated relaxation response was impaired in endothelium intact aortic rings isolated from trained rats (TV and TN) as compared with their matched non-trained groups. TN rats showed reduced ACh-mediated vasodilatation than NTN rats. NO production and bioavailability decreased in thoracic aorta of nandrolone-treated rats in relation to their matched non-trained group (NTN vs. NW

TN vs. TV). ROS production and tunica media thickness were increased in TN rats when compared with TV rats. These findings indicate that high doses of nandrolone combined with strenuous RT affect NO bioavailability and might induce endothelial dysfunction and arterial morphological alterations.
Keywords Nandrolone
Resistance training
Vasodilatation
Nitric oxide
Reactive oxygen species
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
Grant number FAPESP: 07/57380-9
Date 2018
Published in Steroids. New York, v. 131, p. 41456, 2018.
ISSN 0039-128X (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent jul/13
Origin http://dx.doi.org/10.1016/j.steroids.2017.12.013
Access rights Closed access
Type Article
Web of Science ID WOS:000425556100002
URI https://repositorio.unifesp.br/handle/11600/54081

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