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dc.contributor.authorBuri, Marcus V. [UNIFESP]
dc.contributor.authorVieira Torquato, Heron F. [UNIFESP]
dc.contributor.authorBarros, Carlos Castilho
dc.contributor.authorIde, Jaime S.
dc.contributor.authorMiranda, Antonio [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar J. [UNIFESP]
dc.date.accessioned2020-06-26T16:30:33Z
dc.date.available2020-06-26T16:30:33Z
dc.date.issued2017
dc.identifierhttp://dx.doi.org/10.1002/jcb.25844]
dc.identifier.citationJournal Of Cellular Biochemistry. Hoboken, v. 118, n. 7, p. 1764-1773, 2017.
dc.identifier.issn0730-2312
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53623
dc.description.abstractSeveral reports described different modes of cell death triggered by antimicrobial peptides (AMPs) due to direct effects on membrane disruption, and more recently by apoptosis and necrosis-like patterns. Cytotoxic curves of four beta-hairpin AMPs (gomesin, protegrin, tachyplesin, and polyphemusin) were obtained from several human leukemic lineages and normal monocytes and Two cell lines were then selected based on their cytotoxic sensitivity. One was sensitive to AMPs (K562) and the other resistant (KG-1) and their effect compared between these lineages. Thus, these lineages were chosen to further investigate biological features related with their cytotoxicities to AMPs. Stimulation with AMPs produced cell death, with activation of caspase-3, in K562 lineage. Increase on the fluidity of plasmatic membrane by reducing cholesterol potentiated cytotoxicity of AMPs in both lineages. Quantification of internal and external gomesin binding to the cellular membrane of both K562 and KG-1 cells showed that more peptide is accumulated inside of K562 cells. Additionally, evaluation of multi-drug resistant pumps activity showed that KG-1 has more activity than K562 lineage. A comparison of intrinsic gene patterns showed great differences between K562 and KG-1, but stimulation with gomesin promoted few changes in gene expression patterns. Differences in internalization process through the plasma membrane, multidrug resistance pumps activity, and gene expression pattern are important features to AMPs regulated cell death. (C) 2016 Wiley Periodicals, Inc.en
dc.description.sponsorshipINFAR/UNIFESP
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipFAPESP
dc.format.extent1764-1773
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal Of Cellular Biochemistry
dc.rightsACESSO RESTRITO
dc.subjectANTIMICROBIAL PEPTIDEen
dc.subjectCELL DEATHen
dc.subjectMEMBRANE FLUIDITYen
dc.subjectLYSOSOMAL DEGRADATIONen
dc.subjectGENE EXPRESSIONen
dc.subjectMULTI-DRUG RESISTANCEen
dc.titleComparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of beta-Hairpin Antimicrobial Peptidesen
dc.typeArtigo
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, R Tres de Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Pelotas, Dept Nutr, R Gomes Carneiro 1, BR-96010610 Pelotas, RS, Brazil
dc.description.affiliationYale Univ, Sch Med, Dept Psychiat, New Haven, CT 06519 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Biofis, R Tres de Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Av Dr Candido Xavier de Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, R Tres de Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biofis, R Tres de Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sponsorshipIDINFAR/UNIFESP
dc.description.sponsorshipIDFAPESP: 2013/09068-72011
dc.description.sponsorshipIDFAPESP: 2011/17584-0
dc.description.sponsorshipIDCNPq: 473797/2013-5
dc.description.sponsorshipIDFAPESP: 2012/20989-4
dc.identifier.doi10.1002/jcb.25844
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000403051700016
dc.coverageHoboken
dc.citation.volume118]
dc.citation.issue7]


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