Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts

Date
2017Author
Ridolfi, Daniela M.
Lemes, Ana Paula [UNIFESP]
de Oliveira, Sheila [UNIFESP]
Justo, Giselle Z. [UNIFESP]
Palladino, Marcelly V. [UNIFESP]
Duran, Nelson
Type
ArtigoISSN
0969-0239DOI
10.1007/s10570-017-1362-2Metadata
Show full item recordAbstract
Chitosan/poly(ethylene oxide) (PEO) (5:1) nanofibers with cellulose nanocrystals (CNCs) were produced using an electrospinning technique. The addition of CNCs to the chitosan/PEO solutions allowed the production of uniform fibers (without beads) with a high proportion of chitosan. The fiber diameters were influenced by the concentration of CNCs in the chitosan/PEO solutions. The solutions containing 10% (w/w) of CNCs produced thinner fibers compared to solutions containing 5% (w/w) of CNCs. Thermogravimetric analysis indicated that the nanofibers were thermally stable, despite the CNCs having an effect on the PEO decomposition. Results from the cell assay in cultures of 3T3 fibroblasts indicated that the chitosan/PEO nanofibers (with 10% CNCs) promoted cell attachment with changes in the cytoskeletal organization. The results obtained in this work highlight the favorable effect of CNCs in electrospinning of chitosan/PEO. As expected, the influence of nanofibers on 3T3 fibroblasts F-actin and b-tubulin network revealed alterations in cytoskeleton, leading to changes in cell morphology and spreading.
Citation
Cellulose. Dordrecht, v. 24, n. 8, p. 3353-3365, 2017.Keywords
Cellulose nanocrystalsChitosan
Poly(ethylene oxide)
Electrospinning
Nanofibers
Tissue engineering scaffolds
Sponsorship
Conselho Nacional de Desenvolvimento Científico (CNPq)Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
INCT Functional Complex Materials INOMAT (MCT/CNPq)
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