First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells

Data
2017Autor(a)
Marques, E. S.
Tsuboy, M. S. F.
Carvalho, J. C. T.
Rosa, P. C. P.
Perazzo, F. F. [UNIFESP]
Gaivao, I. O. M.
Maistro, E. L.
Tipo
ArtigoISSN
1676-5680DOI
10.4238/gmr16039700Metadado
Mostrar registro completoResumo
Euterpe oleracea Mart., popularly known as "acai", is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a] pyrene, used as DNA-damaging agents.
Citação
Genetics And Molecular Research. Ribeirao Preto, v. 16, n. 3, p. -, 2017.Palavras-chave
Acai oilArecaceae
Comet assay
HepG2 cells
Micronucleus test
Agência(s) de Fomento
FAPESP - Fundação de Amparo a Pesquisa do Estado de São PauloCAPES
Coleções
- ICAQF - Artigos [1140]