Genetic Diversity of HIV-1 Gene vif Among Treatment-Naive Brazilians

Genetic Diversity of HIV-1 Gene vif Among Treatment-Naive Brazilians

Author Villanoval, Fabiola Google Scholar
Barreiros, Marta Google Scholar
Janini, Luiz Mario Autor UNIFESP Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Leal, Elcio Google Scholar
Abstract HIV-1 has the Vif protein, which binds to human antiviral proteins APOBEC3 to form complexes to be degraded by cellular proteolysis. To further explore HIV-1 diversity at the population level, we analyzed blood samples from 317 treatment-naive patients in Brazil. In this study, we explored the correlations of Vif polymorphisms with clinical parameters of the patients and found that mutation K22H is associated with low CD4(+) cell counts and higher viral loads. Phylogenetic analysis of the vif gene indicated that subtype B was predominant in approximate to 77% (243/317) of the patients, followed by HIV-1F approximate to 18% (56/317), and subtype C approximate to 4% (12/317)

five samples were BF recombinants (approximate to 1% of patients), and one was an AG recombinant. On the basis of the vif gene, we detected the presence of one AG and several previously unknown BF intersubtypes in this population. The global mean diversity, measured by pairwise distances, was 0.0931 +/- 0.0006 among sequences of subtype B (n=243), whereas the mean diversity of subtype C sequences (n=12) was 0.0493 +/- 0.001 and that of subtype F (n=56) was 0.050 +/- 0.001.
Keywords HIV-1
gene vif
diversity
hypermutation
recombination
AG intersubtype
Language English
Date 2017
Published in Aids Research And Human Retroviruses. New Rochelle, v. 33, n. 9, p. 952-959, 2017.
ISSN 0889-2229 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert, Inc
Extent 952-959
Origin http://dx.doi.org/10.1089/aid.2016.0230
Access rights Closed access
Type Article
Web of Science ID WOS:000408495200011
URI http://repositorio.unifesp.br/handle/11600/51336

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