Mutagenicity profile of atmospheric particulate matter in a small urban center subjected to airborne emission from vehicle traffic and sugar cane burning

Mutagenicity profile of atmospheric particulate matter in a small urban center subjected to airborne emission from vehicle traffic and sugar cane burning

Author Alves, Debora Kristina M. Google Scholar
Kummrow, Fabio Autor UNIFESP Google Scholar
Cardoso, Arnaldo A. Google Scholar
Morales, Daniel A. Google Scholar
Umbuzeiro, Gisela A. Google Scholar
Abstract Atmospheric particulate matter (PM) is genotoxic and recently was classified as carcinogenic to humans by the International Agency for Research on Cancer. PM chemical composition varies depending on source and atmospheric conditions. The Salmonella/microsome assay is the most used mutagenicity test and can identify the major chemical classes responsible for observed mutagenicity. The objective of this work was to characterize the mutagenicity of PM samples from a countryside city, Limeira, Brazil, which is influenced by heavy traffic and sugar cane biomass burning. Six samples of total PM were collected. Air mass backward trajectories were calculated. Organic extracts were assayed using the Salmonella/microsome microsuspension mutagenicity assay using TA98, YG1041, and TA1538, with and without metabolic activation (S9). YG1041 was the most sensitive strain and mutagenicity reached 9,700 revertants per m(3) without metabolic activation. Potency for TA1538 was higher than TA98, indicating that this strain should be considered in air mutagenicity studies. The increased response to YG1041 relative to TA98, and the decreased response with S9, suggests that nitroaromatics are the major contributors. Limeira is among the most mutagenic cities in the world. High mutagenicity in Limeira seems to occur when the air mass from the area of sugarcane production is mixed with air from the region impacted by anthropogenic activities such as traffic. An increase in the formation of nitro-polycyclic aromatic hydrocarbons may result from longer contact time between the aromatic compounds and the atmosphere with high NOx and ozone concentration, although more studies are required to confirm this hypothesis. Environ. Mol. Mutagen. 57:41-50, 2016. (c) 2015 Wiley Periodicals, Inc.
Keywords Salmonella
Air PollutionPolycyclic Aromatic-Hydrocarbons
Bioassay-Directed Fractionation
Diesel Exhaust Particles
Salmonella Mutagenicity
Ambient Air
Tester Strains
Microsuspension Assay
Organic Material
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [471386/2008-1]
Grant number CNPq: 471386/2008-1
Date 2016
Published in Environmental And Molecular Mutagenesis. Hoboken, v. 57, n. 1, p. 41-50, 2016.
ISSN 0893-6692 (Sherpa/Romeo, impact factor)
Publisher Wiley-blackwell
Extent 41-50
Access rights Closed access
Type Article
Web of Science ID WOS:000368700300005

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