Author |
Fiorindo Faria, Danilo Jose
![]() ![]() Simoes, Manuel de Jesus ![]() ![]() Teixeira, Luciana Cristina ![]() ![]() Pinheiro Faria, Angela Tucci ![]() Simoes Ulhoa Cintra, Alvaro Edmundo ![]() ![]() Martins, Jose Luiz ![]() ![]() |
Abstract | PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR Group B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p <= 0.05*. RESULTS: 81 fetuses were harvested. The number of fetuses number of ARMs mean (Delta%) (+/- SD) were determined to be, respectively: ADR -41[29 65%(+/- 37%)] versus FA+ADR - 40[04 16%(+/- 36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (mu m) of ASSE (+/- SD) and IE (+/- SD) were measured, respectively: ADR -[25.98(+/- 0.74) and 19.48(+/- 1.68)] versus FA+ADR -[24.74(+/- 0.91) and 24.80(+/- 0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced. |
Keywords |
Anal Canal
Anus Imperforate Doxorubicin Folic Acid RatsNeural-Tube Defects Embryonic-Development Hindgut Development Mouse Model Supplementation Notochord Ethylenethiourea Expression Pregnancy Anomalies |
Language | English |
Date | 2016 |
Published in | Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016. |
ISSN | 0102-8650 (Sherpa/Romeo, impact factor) |
Publisher | Acta cirurgica brasileira |
Extent | 22-27 |
Origin |
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Access rights | Open access ![]() |
Type | Article |
Web of Science ID | WOS:000369657900004 |
SciELO ID | S0102-86502016000100022 (statistics in SciELO) |
URI | http://repositorio.unifesp.br/handle/11600/49619 |
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