Hpv genotyping and p16 expression in xingu indigenous park, brazil

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Freitas, V. G. [UNIFESP]
Focchi, G. R. [UNIFESP]
Pereira, E. R. [UNIFESP]
Levi, J. E.
Speck, N. M. G. [UNIFESP]
Ribalta, J. C. [UNIFESP]
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The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec (R) Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 +/- 15.89 years) and parity ranged from 1-11 (5.92 +/- 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant
it was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotype
however, more studies are necessary to further our understanding of this restricted group.
Genetics And Molecular Research. Ribeirao preto, v. 15, n. 3, p. UNSP 15036840, 2016.