Effects of hyperprolactinemia on the tibial epiphyseal plate of mice treated with sex hormones

Effects of hyperprolactinemia on the tibial epiphyseal plate of mice treated with sex hormones

Author Wolff, Roberta Bastos Autor UNIFESP Google Scholar
Gomes, Regina Célia Teixeira Autor UNIFESP Google Scholar
do Amaral, Vinicius C. Google Scholar
da Silva, Priscilla L. Google Scholar
Simoncini, Tommaso Google Scholar
Prosdocimi, Fabio Cesar Google Scholar
Simoes, Ricardo Santos Google Scholar
Simões, Manuel de Jesus S. Autor UNIFESP Google Scholar
Baracat, Edmund Chada Google Scholar
Soares Júnior, José Maria Autor UNIFESP Google Scholar
Abstract The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the tibial epiphyseal plate of hormone-treated oophorectomized mice. For this purpose, 18 animals with intact ovaries were allocated to two groups, M (metoclopramide) and V (vehicle). One hundred and eight oophorectomized animals were allocated to 12 subgroups: Oophx/V (vehicle)

Ooph/M (metoclopramide)

Oophx/V+E (vehicle+estradiol)

Oophx/M+E (metoclopramide+estradiol)

Oophx/V+P (vehicle+progesterone)

Oophx/M+P (metoclopramide+progesterone)

Oophx/V+T (vehicle+testosterone)

Oophx/M+T (metoclopramide+testosterone)

Oophx/V+E+P (Vehicle+estradiol+progesterone)

Oophx/M+E+P (metoclopramide+estradiol+progesterone)

Oophx/V+E+P+T (vehicle+estradiol+progesterone+testosterone)

Oophx/M+E+P+T (metoclopramide+estradiol+progesterone+testosterone). After a 50-day treatment was performed histomorphometric and immunohistochemical cell death analysis. In the epiphyseal plate of the hyperprolactinemic and/or oophorectomized animals, cell proliferation and bone formation decreased, inducing intensified cell death. In the sex steroid-treated animals, estrogen boosted cell proliferation

progesterone, bone formation and testosterone, both cell proliferation and bone formation. These findings suggest that oophorectomy and hyperprolactinemia changed epiphyseal plate morphology causing cartilage degeneration. Treatment with combined sex steroids may diminish such deleterious effects.
Keywords Estrogens
Hormone Replacement Therapy
OsteoporosisProlactin Receptor Expression
Uterine Prolactin
Trabecular Bone
Knockout Mice
Language English
Date 2016
Published in Gynecological Endocrinology. Abingdon, v. 32, n. 1, p. 30-33, 2016.
ISSN 0951-3590 (Sherpa/Romeo, impact factor)
Publisher Cuba Editora
Extent 30-33
Origin https://doi.org/10.3109/09513590.2015.1068753
Access rights Closed access
Type Article
Web of Science ID WOS:000368719300008
URI http://repositorio.unifesp.br/handle/11600/49202

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