Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease

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dc.contributor.author Pezato, Rogerio [UNIFESP]
dc.contributor.author Swierczynska-Krepa, Monika
dc.contributor.author Niankowska-Mogilnicka, Ewa
dc.contributor.author Holtappels, Gabriele
dc.contributor.author De Ruyck, Natalie
dc.contributor.author Sanak, Marek
dc.contributor.author Derycke, Lara
dc.contributor.author Van Crombruggen, Koen
dc.contributor.author Bachert, Claus
dc.contributor.author Perez-Novo, Claudina A.
dc.date.accessioned 2018-07-26T17:30:31Z
dc.date.available 2018-07-26T17:30:31Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1016/j.cyto.2015.10.011
dc.identifier.citation Cytokine. London, v. 77, p. 157-167, 2016.
dc.identifier.issn 1043-4666
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/46094
dc.description.abstract Background: Systemic reactions are related to the pathogenesis of Aspirin Exacerbated Respiratory Disease (AERD). With this work we wanted to study the changes in the systemic levels of inflammatory mediators in both baseline and after oral aspirin challenge in patients with and without AERD. Methods: Patients with nasal polyposis and asthma with AERD (n = 20) and without (n = 18) were orally challenged with aspirin in a single-blind placebo controlled study. Serum samples and urine were collected before and 6 h after placebo and aspirin oral challenges. Serum levels of inflammatory mediators were assayed by using the Luminex technology and ELISA. The concentrations of 9-alpha, 11-beta prostaglandin F-2, and leukotriene E-4 (uLTE(4)) were measured in urine samples by ELISA. The expression of T-cell surface markers was analyzed in peripheral blood mononuclear cells isolated before and after the challenges. Results: AERD patients showed significantly higher baseline levels of s-IL-5R-alpha, uLTE(4) and percentage of CD4(+)CD25(+)CD127(pos) and CD4(+)CD45RA(-)CD45RO(+) but decreased levels of TGF-beta(1) and number of CD4(+)CD25(+)CD127(neg) cells. Aspirin challenge induced the release of uLTE4, IL-6 and increased the number of CD4(+)CD45RA(-)CD45RO(+) memory T-cells only in AERD patients but failed to reduce the levels of sCD40L as observed in non-AERD subjects. Further, IL-8 and sIL-5R-alpha levels directly correlated with the PD(20)ASA and the effects of aspirin on IL-6 and number of memory T-cells was more pronounced in subjects showing more strong reaction (bronchial and nasal). Conclusions: AERD patients have a differential baseline inflammatory pattern that supports the role inflammation as underlying mechanism of the disease. Systemic response to oral aspirin challenge was related to an increase in serum IL-6 and the number of circulating memory T-cells in AERD patients. (C) 2015 Elsevier Ltd. All rights reserved. en
dc.description.sponsorship Flemish Research Board (FWO) [FWO08-PDO-117]
dc.description.sponsorship Flemish Scientific Research Board (FWO) [A12/5-HB-KH3, 1841713N, G039412N]
dc.description.sponsorship Interuniversity Attraction Poles (IAP) Project [P7/30]
dc.description.sponsorship Jagiellonian University [K/ZDS/000362]
dc.format.extent 157-167
dc.language.iso eng
dc.publisher Academic Press Ltd- Elsevier Science Ltd
dc.relation.ispartof Cytokine
dc.rights Acesso restrito
dc.subject Aspirin Exacerbated Respiratory Disease en
dc.subject Interleukin 6 en
dc.subject Memory T-cells en
dc.subject Oral aspirin challenge en
dc.subject Systemic reaction after aspirin challenge en
dc.subject Interleukin 8 en
dc.subject IL-5 receptor alpha en
dc.subject TGF-beta(1)Sensitive Patients en
dc.subject Bronchial-Asthma en
dc.subject T-Cells en
dc.subject Moderate Asthma en
dc.subject Hypersensitivity en
dc.subject Challenge en
dc.subject Activation en
dc.subject Tgf-Beta-1 en
dc.subject Association en
dc.subject Leukocytes en
dc.title Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease en
dc.type Artigo
dc.description.affiliation [Pezato, Rogerio
dc.description.affiliation Holtappels, Gabriele
dc.description.affiliation De Ruyck, Natalie
dc.description.affiliation Derycke, Lara
dc.description.affiliation Van Crombruggen, Koen
dc.description.affiliation Bachert, Claus
dc.description.affiliation Perez-Novo, Claudina A.] Ghent Univ Hosp, Dept Otorhinolaryngol, Upper Airways Res Lab, Ghent, Belgium
dc.description.affiliation [Pezato, Rogerio] Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Sao Paulo, Brazil
dc.description.affiliation [Swierczynska-Krepa, Monika] Medex, Out Patient Allergy Clin, Bielsko Biala, Poland
dc.description.affiliation [Niankowska-Mogilnicka, Ewa
dc.description.affiliation Sanak, Marek] Jagiellonian Univ, Sch Med, Dept Med, Krakow, Poland
dc.description.affiliationUnifesp Dept. of Otorhinolaryngology, Head and Neck Surgery, Federal University of São Paulo, São Paulo, Brazil
dc.description.sponsorshipID This work was supported by a grant to Dr. Claudina Perez-Novo from the Flemish Research Board (FWO Post-doctoral mandate, Nr. FWO08-PDO-117), grants to Prof. Claus Bachert from the Flemish Scientific Research Board (FWO Nr. A12/5-HB-KH3, FWO mandate: 1841713N and FWO research project: G039412N), the Interuniversity Attraction Poles (IAP) Project P7/30 and the statutory grant from the Jagiellonian University (K/ZDS/000362) awarded to Dr. Monika Swierczynska-Krepa.
dc.identifier.doi 10.1016/j.cyto.2015.10.011
dc.description.source Web of Science
dc.identifier.wos WOS:000366540500020



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