Her2/neu expression by reverse transcriptase-polymerase chain reaction in the peripheral blood of prostate cancer patients

Her2/neu expression by reverse transcriptase-polymerase chain reaction in the peripheral blood of prostate cancer patients

Author Fantinato, Ana Paula Autor UNIFESP Google Scholar
Tobias-Machado, Marcos Google Scholar
Fonseca, Fernando Google Scholar
Pinto, Jorge Luiz Freire Autor UNIFESP Google Scholar
Wroclawski, Marcelo Langer Google Scholar
Wroclawski, Eric Google Scholar
Verotti, Mauricio Google Scholar
del Giglio, Auro Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background/aims: Evaluation of Her2/neu expression in the peripheral blood mononuclear cell fraction of prostate cancer patients by RT-PCR may afford an opportunity for the detection of circulating tumor cells and thus serve as a marker of micrometastatic disease.Methods: We studied Her2/neu expression by reverse transcriptase-polymerase chain reaction in peripheral blood mononuclear cell fraction samples of 21 controls and serially in 43 patients with prostate cancer.Results: None of the 21 controls expressed Her2/neu whereas 23.25% (95% Cl, 11.75-38.63) of the patients were positive at entry into the study, and 65.11% (95% CI, 49.07-78.99) of them had at least one positive result during the follow-up period. Her2/neu positivity at study entry did not correlate significantly with PSA level, Gleason score, clinical stage or time to PSA progression. When we analyzed only patients with advanced disease, we observed a trend towards a shorter time to PSA progression in patients with at least one positive Her2/neu result during the follow-up (log-rank test, P = 0.08).Conclusions: We conclude that Her2/neu expression in the peripheral blood mononuclear cell fraction of prostate cancer patients is frequent and therefore this assay may potentially be useful to detect the presence of micrometastatic disease in men with prostate cancer and for monitoring patients enrolled in trastuzumab-based therapeutic protocols.
Keywords erB-2
neoplasm circulating cells
polymerase chain reaction
prostatic neoplasms
receptor
Language English
Date 2007-09-01
Published in Tumori. Rome: Pensiero Scientifico Editor, v. 93, n. 5, p. 467-472, 2007.
ISSN 0300-8916 (Sherpa/Romeo, impact factor)
Publisher Pensiero Scientifico Editor
Extent 467-472
Origin http://www.tumorijournal.com/article/her2neu-expression-by-reverse-transcriptase-polymerase-chain-reaction-in-the-peripheral-blood-of-prostate-cancer-patients
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000253730300010
URI http://repositorio.unifesp.br/11600/45713

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