Interleukin-6-174 G/C promoter polymorphism and nasal polyposis

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dc.contributor.author Kosugi, Eduardo Macoto [UNIFESP]
dc.contributor.author Camargo-Kosugi, Cintia Meirelles de [UNIFESP]
dc.contributor.author Maurice Weckx, Luc Louis [UNIFESP]
dc.contributor.author Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.author Gregorio, Luiz Carlos [UNIFESP]
dc.date.accessioned 2018-06-18T11:54:41Z
dc.date.available 2018-06-18T11:54:41Z
dc.date.issued 2009-12-01
dc.identifier http://www.rhinologyjournal.com/Rhinology_issues/807.pdf
dc.identifier.citation Rhinology. Utrecht: Int Rhinologic Soc, v. 47, n. 4, p. 400-404, 2009.
dc.identifier.issn 0300-0729
dc.identifier.uri http://repositorio.unifesp.br/11600/45527
dc.description.abstract Introduction: Nasal polyposis is a chronic disease with unknown etiopathogenesis, although inflammatory mechanisms seem to play a role. One of several inflammatory mediators linked to nasal polyposis is Interleukin-6, which has a single. nucleotide polymorphism -174 G/C that seems to promote an inflammatory reaction.Objective: To compare the prevalence of the -174 G/C single nucleotide polymorphism between a group of patients with nasal polyposis and a control group.Method. Cross-sectional study with two groups (thirty two patients with nasal polyposis and fifty five controls) to investigate the -174 G/C polymorphism in blood samples. Asthma, aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with forward primer 5'-ATGCCAAGTGCTGAGTCACTA-3' and reverse primer 5'-GGAAAATCCCACATTTGATA-3', amplifying a 226-bp DNA fragment that contained the 174 position. The amplified fragment can be cleaved by restriction enzyme NlaIII when the -174 position presented the C allele in two fragments of 117 and 109-bp, visualized by electrophoresis, classifying participants in GG, GC and CC.Results: In the nasal polyposis group, 65.62% of the patients had the GG genotype, while in the control group only 41.82% had the GG genotype, a statistically significant difference, with an odds ratio of 2.65.Conclusion: The -174 GG genotype was found more frequency in nasal polyposis patients than in controls, when asthma, aspirin intolerance and atopy were excluded. en
dc.format.extent 400-404
dc.language.iso eng
dc.publisher Int Rhinologic Soc
dc.relation.ispartof Rhinology
dc.rights Acesso aberto
dc.subject nasal polyps en
dc.subject interleukin-6 en
dc.subject genetic polymorphism en
dc.subject restriction fragment length polymorphism en
dc.subject single nucleotide polymorphism en
dc.title Interleukin-6-174 G/C promoter polymorphism and nasal polyposis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, EPM, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Dept Ginecol, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Ginecol, EPM, Sao Paulo, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000273234200015



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