Preclinical investigation of the retinal biocompatibility of six novel vital dyes for chromovitrectomy

Preclinical investigation of the retinal biocompatibility of six novel vital dyes for chromovitrectomy

Author Rodrigues, Eduardo Buchele Autor UNIFESP Google Scholar
Penha, Fernando Marcondes Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Costa, Elaine de Paula Fiod Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Bottos, Juliana Autor UNIFESP Google Scholar
Freymuller, Edna Autor UNIFESP Google Scholar
Furlani, Bruno de Albuquerque Autor UNIFESP Google Scholar
Meyer, Carsten H. Autor UNIFESP Google Scholar
Magalhães, Octaviano Autor UNIFESP Google Scholar
Lima-Filho, Acacio Alves Souza Autor UNIFESP Google Scholar
Safatle, Angélica Mendonça de Vaz Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Bonn
Universidade de São Paulo (USP)
Abstract Purpose: To investigate the retinal biocompatibility of six novel vital dyes for chromovitrectomy.Methods: An amount of 0.05 mL of 0.5% and 0.05% light green (LG), fast green (FG), Evans blue (EB), brilliant blue (BriB), bromophenol blue (BroB), or indigo carmine (IC) was injected intravitreally in the right eye, whereas in the left eye balanced salt solution was applied for control in rabbits' eyes. Clinical examination, fluorescein angiography, histology with light microscopy, and transmission electron microscopy were performed after 1 and 7 days. Retinal cell layers were evaluated for morphologic alterations and number of cells. The electroretinographic changes were assessed at baseline, 24 hours and 7 days.Results: Fluorescein angiography disclosed hypofluorescent spots only in the 0.5% EB group. Light microscopy and transmission electron microscopy disclosed slight focal morphologic changes in eyes exposed to 0.05% IC, FG, BriB, similar to the control at 1 and 7 days. In the lower dose groups, EB, LG, and BroB caused substantial retinal alterations by light microscopy. At the higher dose, BroB and EB produced diffuse cellular edema and vacuolization within the ganglion cells, bipolar cells, and photoreceptors. FG and IC at 0.5% caused slight retinal alterations similar to balanced salt solution injection. LG at 0.5% caused diffuse vacuolization of bipolar cells after 1 and 7 days. Injection of 0.5% EB caused a significant decrease in neuroretinal cell counts in comparison to control eyes in the 7-day examination (P < 0.05). Electroretinography revealed intermittent prolonged latency and decreased amplitude in eyes injected with 0.5% EB, LG, BriB, and BroB, while at the lower dose, only LG and EB induced few functional changes.Conclusion: The progressive order of retinal biocompatibility, from safest to most toxic, was IC, FG, BriB, BroB, LG, EB.
Keywords chromovitrectomy
indocyanine green
fast green
vital dye
trypan blue
brilliant blue
bromophenol blue
Language English
Sponsor German Academic Exchange Service (DAAD)
Pan-American Association of Ophthalmology-Pan-American Ophthalmological Foundation (PAAO/PAOF)
Vision Institute-UNIFESP
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-04-01
Published in Retina-the Journal Of Retinal And Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 29, n. 4, p. 497-510, 2009.
ISSN 0275-004X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 497-510
Access rights Closed access
Type Article
Web of Science ID WOS:000265203800012

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