Adjuvant effect of the propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene
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2006-01-01
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Mussalem, Juliana Sekeres [UNIFESP]
Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]
Squaiella, Carla Cristina [UNIFESP]
Ananias, Renata Zeigler [UNIFESP]
Braga, Eleni Goncalves [UNIFESP]
Rodrigues, Mauricio Martins [UNIFESP]
Longo-Maugeri, Ieda Maria [UNIFESP]
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In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene-encoding catalytic domain of Trypanosoma cruzi (T cruzi) trans-sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited Immoral, cell-mediated and protective immune responses against T cruzi infection. In this study we describe that both P acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans-sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN-gamma synthesis by CD4(+) T cells. The most important fact was that treatment with P acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T cruzi challenge. These data suggest that P acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T cruzi infection.
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Microbiology And Immunology. Tokyo: Center Academic Publ Japan, v. 50, n. 4, p. 253-263, 2006.