Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer

Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer

Author Lima, Eleonidas Moura Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Khayat, Andre Salim Google Scholar
Assumpção, Paulo Pimentes de Autor UNIFESP Google Scholar
Bello, Maria Jose Google Scholar
Rey, J.a. Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Casartelli, Carla Google Scholar
Institution Universidade Federal do Piauí Colegiado de Biomedicina
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Pará Instituto de Ciências Biológicas Laboratório de Citogenética Humana
Hospital João de Barros Barreto Serviço de Cirurgia
Instituto de Investigaciones Biomedicas
Universidade de São Paulo (USP)
Abstract Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.
Keywords DNA methylation
Gastric cancer
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FINEP/CT-INFRA: 0927-03
Date 2008-06-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 6, p. 539-543, 2008.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 539-543
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000258766600017
SciELO ID S0100-879X2008000600017 (statistics in SciELO)

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