Alterations of spermatogenesis in etoposide-treated rats: A stereological study

Alterations of spermatogenesis in etoposide-treated rats: A stereological study

Author Freitas, Francisca Estela Lima Autor UNIFESP Google Scholar
Mori, Flora Cordeiro Autor UNIFESP Google Scholar
Lucas, Sandra Regina Rodrigues Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fed Univ Acre
Univ Estadual Paulista
Abstract The etoposide is an anticancer drug that interacts with topoisomerase II. Thirty-day-old rats received intraperitonially 2mg/kg of etoposide for 30 consecutive days. Their testes were analyzed in the adult phase under light microscopy according to histomorphometric and stereological parameters. Random 3mum-thick-paraplast sections of testis were stained with periodic acid-Schiff reaction and Harris' hematoxylin method. Serum testosterone level and reproductive performance were also investigated. The results showed an accentuated decrease in the frequency of germinal lineage cell types and differentiated spermatogonia were the most affected cell types. Morphometric and stereological testicular parameters exhibited highly, significant reductions in adult etoposide-treated rats. Their reproductive performance diminished but their serum testosterone level was not significantly altered. The mortality frequency of the progenies was 100%.
Keywords etoposide
seminiferous epithelium
Language English
Date 2002-05-01
Published in Interciencia. Caracas: Interciencia, v. 27, n. 5, p. 227-235, 2002.
ISSN 0378-1844 (Sherpa/Romeo, impact factor)
Publisher Interciencia
Extent 227-235
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000175969700003

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