Acquired immune responses to the N- and C-terminal regions of Plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria

Acquired immune responses to the N- and C-terminal regions of Plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria

Author Soares, Irene da Silva Autor UNIFESP Google Scholar
Levitus, Gabriela Google Scholar
Souza, José M. Google Scholar
Del Portillo, Hernando A. Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Institution INST EVANDRO CHAGAS
FED UNIV PARA
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract In this study, we evaluated the naturally acquired immune response to Plasmodium vivax merozoite surface protein 1 (PvMSP1) in individuals with recent clinical episodes of malaria from the state of Para, Brazil. Ten recombinant proteins representing the first 682 amino acids (aa) of the N-terminal region and one representing the final 111 aa of the C-terminal region were expressed in Escherichia coli as glutathione S-transferase fusion proteins. Both of these regions have been suggested as candidates for development of a vaccine against Plasmodium sp. The total frequencies of individuals with antibodies and cellular immune responses to PvMSP1 were high (83.8 and 75%, respectively). The recombinant proteins representing the N- and C-terminal regions were recognized by 51.4 and 64.1% of sera, respectively. The frequency of responders to the C-terminal region increased according to the number of previous malaria episodes, reaching 83.3% after four episodes. Cellular immune response was measured by in vitro proliferation and gamma interferon production. Peripheral blood mononuclear cells of 75 adn 47.2% of individuals proliferated in response to stimulation by the N- and C-terminal regions, respectively. Also, we found that one protein representing the N terminus and a second representing the C terminus of PvMSP1 stimulated 54.5% of individuals to secrete gamma interferon. We concluded that PvMSP1 is immunogenic to a large proportion of individuals exposed to malaria. Our results also suggested that the C-terminal region of PvMSP1 containing the two epidermal growth factor-like domains is particularly immunogenic to antibodies and T cells during natural infection in humans.
Language English
Date 1997-05-01
Published in Infection And Immunity. Washington: Amer Soc Microbiology, v. 65, n. 5, p. 1606-1614, 1997.
ISSN 0019-9567 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 1606-1614
Origin http://iai.asm.org/content/65/5/1606.abstract
Access rights Closed access
Type Article
Web of Science ID WOS:A1997WW39800006
URI http://repositorio.unifesp.br/11600/43960

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