Characterization of a cDNA clone encoding the carboxy-terminal domain of a 90-kilodalton surface antigen of Trypanosoma cruzi metacyclic trypomastigotes
Franco, Fulvia Regina de Souza [UNIFESP]
Paranhos-Baccala, Glaucia S.
Yamauchi, Lucy Megumi [UNIFESP]
Yoshida, Nobuko [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
Is part ofInfection And Immunity
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We have cloned and sequenced a cDNA for a metacyclic trypomastigote-specific glycoprotein, with a molecular mass of 90 kDa, termed MTS-gp90. By immunoblotting, antibodies to the MTS-gp90 recombinant protein reacted exclusively with a 90-kDa antigen of metacyclic trypomastigotes. The insert of the MTS-gp90 cDNA clone strongly hybridized with a single 3.0-kb mRNA of metacyclic forms, whereas the hybridization signal with epimastigote mRNA was weak and those with RNAs from other developmental stages were negative, indicating that transcription of the MTS-gp90 gene is developmentally regulated. A series of experiments showed that the MTS-gp90 gene is present in multiple copies in the Trypanosoma cruzi genome, arranged in a nontandem manner, and that there are at least 40 copies of the gene per haploid genome. Sequence analysis of recombinant MTS-gp90 revealed 40 to 60% identity at the amino acid level with members of a family of mammalian stage-specific, 85-kDa surface antigens of T. cruzi. However, there are considerable differences in the amino acid compositions outside the homology region.
CitationInfection And Immunity. Washington: Amer Soc Microbiology, v. 61, n. 10, p. 4196-4201, 1993.
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