Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats

Show simple item record Beltrame, Flavia Luciana [UNIFESP] Yamauti, Caroline T. Caneguim, Breno Henrique [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Miraglia, Sandra Maria [UNIFESP] Sasso-Cerri, Estela [UNIFESP] 2018-06-15T17:35:07Z 2018-06-15T17:35:07Z 2012-10-01
dc.identifier.citation Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.
dc.identifier.issn 0213-3911
dc.description.abstract Cimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 1343-1351
dc.language.iso eng
dc.publisher F Hernandez
dc.relation.ispartof Histology And Histopathology
dc.rights Acesso restrito
dc.subject H-2 receptor antagonist en
dc.subject Microvascular density en
dc.subject Vascular atrophy en
dc.subject Cell death en
dc.subject Smooth muscle cell en
dc.title Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats en
dc.type Artigo
dc.contributor.institution Sao Paulo State Univ UNESP
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Sao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliation Fed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.affiliationUnifesp Fed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2006/50324-3
dc.description.sponsorshipID FAPESP: 2006/54776-6
dc.description.sponsorshipID FAPESP: 2007/53207-0
dc.description.sponsorshipID FAPESP: 2010/02409-5
dc.identifier.doi 10.14670/HH-27.1343
dc.description.source Web of Science
dc.identifier.wos WOS:000308583700011


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