Effects of Lopap on human endothelial cells and platelets

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dc.contributor.author Chudzinski-Tavassi, Ana Marisa [UNIFESP]
dc.contributor.author Schattner, M.
dc.contributor.author Fritzen, Marcio [UNIFESP]
dc.contributor.author Pozner, R. G.
dc.contributor.author Reis, C. V.
dc.contributor.author Lourenco, Dayse Maria [UNIFESP]
dc.contributor.author Lazzari, M. A.
dc.date.accessioned 2018-06-15T17:30:23Z
dc.date.available 2018-06-15T17:30:23Z
dc.date.issued 2001-05-01
dc.identifier https://doi.org/10.1159/000048071
dc.identifier.citation Haemostasis. Basel: Karger, v. 31, n. 3-6, p. 257-265, 2001.
dc.identifier.issn 0301-0147
dc.identifier.uri http://repositorio.unifesp.br/11600/43739
dc.description.abstract Severe consumption coagulopathy has been detected in rats after Lopap (a prothrombin activator from Lonomia obliqua caterpillar bristles) infusion and in humans after accidental contact with L. obliqua bristles. However, platelet count and antithrombin (AT) levels were only modestly affected, suggesting that a different form of blood coagulation activation may be involved in this hemorrhagic syndrome. Here we describe that Lopap had no effect on aggregation of washed human platelets induced by several agonists, suggesting that it might not impair platelet function in vivo. AT was able to inhibit the amidolytic activity of thrombin generated by incubation of Lopap with prothrombin in a purified system, which maybe different from that generated by the prothrombinase complex in vivo. The surface expression of both ICAM-1 and E-selectin but not of VCAM-1 was upregulated by Lopap in cultured HUVEC, suggesting that it may behave differently from other mediators, such as thrombin and tumor necrosis factor-alpha. Copyright (C) 2002 S. Karger AG, Basel. en
dc.format.extent 257-265
dc.language.iso eng
dc.publisher Karger
dc.relation.ispartof Haemostasis
dc.rights Acesso aberto
dc.subject Lopap en
dc.subject prothrombin activator en
dc.subject Lonomia obliqua en
dc.subject platelets en
dc.subject ICAM-1 en
dc.subject E-selectin en
dc.subject endothelial cells en
dc.title Effects of Lopap on human endothelial cells and platelets en
dc.type Artigo
dc.rights.license http://www.karger.com/Services/RightsPermissions
dc.contributor.institution FAPESP
dc.contributor.institution Natl Acad Med
dc.contributor.institution Consejo Nacl Invest Cient & Tecn
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation FAPESP, Biochem & Biophys Lab, Butantan Inst, CEPID,Ctr Appl Toxinol, BR-05503900 Sao Paulo, Brazil
dc.description.affiliation Natl Acad Med, Hematol Res Inst, Buenos Aires, DF, Argentina
dc.description.affiliation Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
dc.description.affiliation UNIFESP, Dept Hematol, Sao Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Dept Hematol, Sao Paulo, Brazil
dc.identifier.doi 10.1159/000048071
dc.description.source Web of Science
dc.identifier.wos WOS:000174839200019


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