Effects of Lopap on human endothelial cells and platelets

Effects of Lopap on human endothelial cells and platelets

Author Chudzinski-Tavassi, Ana Marisa Autor UNIFESP Google Scholar
Schattner, M. Google Scholar
Fritzen, Marcio Autor UNIFESP Google Scholar
Pozner, R. G. Google Scholar
Reis, C. V. Google Scholar
Lourenco, Dayse Maria Autor UNIFESP Google Scholar
Lazzari, M. A. Google Scholar
Institution FAPESP
Natl Acad Med
Consejo Nacl Invest Cient & Tecn
Universidade Federal de São Paulo (UNIFESP)
Abstract Severe consumption coagulopathy has been detected in rats after Lopap (a prothrombin activator from Lonomia obliqua caterpillar bristles) infusion and in humans after accidental contact with L. obliqua bristles. However, platelet count and antithrombin (AT) levels were only modestly affected, suggesting that a different form of blood coagulation activation may be involved in this hemorrhagic syndrome. Here we describe that Lopap had no effect on aggregation of washed human platelets induced by several agonists, suggesting that it might not impair platelet function in vivo. AT was able to inhibit the amidolytic activity of thrombin generated by incubation of Lopap with prothrombin in a purified system, which maybe different from that generated by the prothrombinase complex in vivo. The surface expression of both ICAM-1 and E-selectin but not of VCAM-1 was upregulated by Lopap in cultured HUVEC, suggesting that it may behave differently from other mediators, such as thrombin and tumor necrosis factor-alpha. Copyright (C) 2002 S. Karger AG, Basel.
Keywords Lopap
prothrombin activator
Lonomia obliqua
platelets
ICAM-1
E-selectin
endothelial cells
Language English
Date 2001-05-01
Published in Haemostasis. Basel: Karger, v. 31, n. 3-6, p. 257-265, 2001.
ISSN 0301-0147 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 257-265
Origin https://doi.org/10.1159/000048071
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000174839200019
URI http://repositorio.unifesp.br/11600/43739

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