Analysis of ICAM-1 and HLA-DR expression on renal allograft aspirates

Analysis of ICAM-1 and HLA-DR expression on renal allograft aspirates

Author Manfro, Roberto Ceratti Autor UNIFESP Google Scholar
Goncalves, LFS Google Scholar
Rauber, M. Google Scholar
Moura, Luiz Antonio Ribeiro de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract ICAM-1 and HLA-DR expressions were studied on parenchymal cells obtained by aspirative biopsies of renal allografts. Patients were evaluated during 3 months after transplantation. Conventional cytology and immunoperoxidase stainings using anti-ICAM-1 and anti-HLA-DR antibodies were employed. The value of the total corrected increment was significantly higher (p<0.05) during acute rejection episodes than during acute tubular necrosis or stable renal function, The percentage of HLA-DR positive cells were higher in rejection than in stable function periods (p<0.05). The percentage of ICAM-1 positive cells was higher in rejection than in acute tubular necrosis periods (p<0.05). The sensitivities to the diagnosis of rejection were 71.8% (TCI) 68.4% (ICAM-1) and 55.0% (HLA-DR). The specificities were 87.3%, 81.3% and 68.4% respectively. Combinations of the tests resulted in increased sensitivity. We concluded that the use of these monoclonal antibodies improves the sensitivity of conventional aspiration cytology to the diagnosis of acute renal rejection.
Keywords acute rejection
fine needle aspiration biopsy
kidney transplantation
Language English
Date 1996-08-01
Published in Clinical Transplantation. Copenhagen: Munksgaard Int Publ Ltd, v. 10, n. 4, p. 379-383, 1996.
ISSN 0902-0063 (Sherpa/Romeo, impact factor)
Publisher Munksgaard Int Publ Ltd
Extent 379-383
Access rights Closed access
Type Article
Web of Science ID WOS:A1996VH54900010

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