LOW DIHYDROPYRIDINE RECEPTOR DENSITY IN VASA DEFERENTIA OF CASTRATED RATS

LOW DIHYDROPYRIDINE RECEPTOR DENSITY IN VASA DEFERENTIA OF CASTRATED RATS

Author Castillo, CJF Google Scholar
Lafayette, Simone Sette Lopes Autor UNIFESP Google Scholar
Caricati-Neto, Afonso Autor UNIFESP Google Scholar
Sette, Mario Autor UNIFESP Google Scholar
Jurkiewicz, Neide Hyppolito Autor UNIFESP Google Scholar
Garcia, Antonio G. Google Scholar
Jurkiewicz, Aron Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNIV AUTONOMA MADRID
Abstract Radioligand binding studies in crude membrane preparations of vasa deferentia of normal rats, with the 1,4-dihydropyridine (+)-[H-3]-PN200-110 (isradipine) showed typical saturation isotherms. The binding exhibited a K(D) of 259 +/- 60 pM and B(max) of 144 +/- 20 fmol mg-1 protein. The low K(D) and the stereoselective displacement of (+)-[H-3]-PN200-110 binding by (+)- and (-)-PN200-110 and by nifedipine suggests that these tissues contain dihydropyridine receptors probably coupled to voltage-sensitive, L-type calcium channels. In membrane preparations from vasa deferentia from rats castrated 30 days previously the maximum specific binding was 25 +/- 10 fmol mg-1 protein, representing only 11% of total binding; thus, the calculation of reliable K(D) values was not feasible. These findings suggest that a testicular hormone, possibly testosterone, plays an important role in the regulation of dihydropyridine-sensitive, voltage-dependent calcium channels in the rat vas deferens.
Keywords DIHYDROPYRIDINE RECEPTORS
VAS DEFERENS
CALCIUM CHANNELS
CASTRATION
Language English
Date 1992-02-01
Published in British Journal Of Pharmacology. Basingstoke: Stockton Press, v. 105, n. 2, p. 257-258, 1992.
ISSN 0007-1188 (Sherpa/Romeo, impact factor)
Publisher Stockton Press
Extent 257-258
Origin http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.1992.tb14241.x/full
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1992HC75900001
URI http://repositorio.unifesp.br/11600/43174

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