Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis
Magalhaes, Vanda Dolabela de [UNIFESP]
Castilho, Beatriz Amaral de [UNIFESP]
Is part ofBrazilian Journal Of Medical And Biological Research
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The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac(+); another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria.
CitationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 30, n. 3, p. 363-367, 1997.
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