BINDING OF HEPARIN AND COMPOUND-Y TO ENDOTHELIAL-CELLS STIMULATES THE SYNTHESIS OF AN ANTITHROMBOTIC HEPARAN-SULFATE PROTEOGLYCAN
Data
1994-09-01
Autores
Pinhal, Maria Aparecida da Silva 
Dietrich, Carl Peter
Nader, Helena Bonciani
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
The mechanism by which heparin and antithrombotic agents, including a cyclic octaphenolsulfonic acid (compound Y), stimulate the synthesis of an antithrombotic heparan sulfate by endothelial cells in culture was investigated. Compound Y increases the amount of heparan sulfate from the cell surface and secreted to the medium by endothelial cells by three-fold. Binding experiments have shown saturation of the endothelial cell receptors at a concentration of 0.16 mu M for heparin and 2.7 mu M for compound Y. The kinetic binding constants (Ks) for compound Y and heparin were 1,333 nM and 42 nM, respectively. It was also shown that both compounds bind to the same receptors. The Scatchard plots indicated that 1,319 nmoles compound Y and 35 nmoles heparin bound per microgram cell protein, indicating that 40-fold more molecules of compound Y bound to the receptors when compared to heparin. No significant internalization Of the compounds was observed.
Descrição
Citação
Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 9, p. 2191-2195, 1994.