THE KINETICS OF CHONDROITIN 4-SULFATE RELEASE FROM STIMULATED PLATELETS AND ITS RELATION TO THROMBOXANE A(2) FORMATION AND GRANULE SECRETION

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Data
1994-09-01
Autores
Donato, J. L.
Nogueira, Marie Doki [UNIFESP]
Marcondes, S.
Antunes, E.
Nader, Helena Bonciani [UNIFESP]
Dietrich, Carl Peter [UNIFESP]
Denucci, G.
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1. In platelet rich plasma (PRP), chondroitin 4-sulfate release from platelets occurred after stimulation with ADP (5 mu M), collagen (5-10 mu g/ml), or adrenaline (10 mu M). Release started within 60 s and maximum release (0.7-2.0 mg/l) was reached within 180 s. TXA(2) formation and dense granule release reached a maximum within 90 s after stimulation.2. Using washed platelets (1.5 x 10(8) cells/ml), the platelet responses were faster. Release of chondroitin 4-sulfate and TXA(2) started within 20-30 s after thrombin addition (100 mU/ml). Maximum release was reached within 60 s in both cases. Dense granule release started in the first 5 s of stimulation (34.6 +/- 12.4%) reaching maximum secretion (74.4 +/- 8.7%) within 60 s.3. Our results demonstrate that maximal chondroitin 4-sulfate release occurs after the dense granule release reaction in both PRP and washed platelets. This observation suggests that chondroitin 4-sulfate is unlikely to be stored in the dense granules but may be stored in the alpha-granules.
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Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 9, p. 2163-2167, 1994.
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