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dc.contributor.authorVieira, Jose Gilberto Henriques [UNIFESP]
dc.contributor.authorNishida, Sonia Kiyomi [UNIFESP]
dc.contributor.authorKasamatsu, Teresa Sayoko [UNIFESP]
dc.contributor.authorAmarante, E. C.
dc.contributor.authorKunii, Ilda Sizue [UNIFESP]
dc.date.accessioned2018-06-15T14:28:05Z
dc.date.available2018-06-15T14:28:05Z
dc.date.issued1994-10-01
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 10, p. 2379-2382, 1994.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/11600/43046
dc.description.abstractParathyroid hormone (PTH) is a linear peptide of 84 amino acids that is found in serum mainly in the form of carboxyl-terminal fragments. The biological activity of PTH depends on the presence of the amino-terminal portion and in circulation is limited to the intact molecule. We describe an immunofluorometric assay for the measurement of PTH-(1-84) based on a chicken egg yolk-derived amino-terminal antibody bound to microtiter plates by an anti-chicken Ig monoclonal antibody. As tracer antibody we employed a Europium-labelled carboxyl-terminal specific monoclonal antibody produced from a mouse immunized with hPTH-(53-84)-BSA conjugate. The assay included an initial overnight incubation of the sample and the solid phase-bound amino-terminal antibody, followed by washing and addition of the tracer antibody, and an additional two hours of incubation prior to fluorescence reading. The least-detectable dose was in the order of 2.5 pg/ml and preliminary studies in 40 normal adults showed values in the range of 4 to 70 pg/ml; for 12 patients with surgery-proven primary hyperparathyroidism values ranged from 109 to 743 pg/ml and for 34 patients with humoral hypercalcemia of malignancy from 2.5 to 66 pg/ml. We conclude that this assay, with its increased sensitivity and specificity, will be a valuable tool in the study of PTH secretion in normal and pathological situations.en
dc.format.extent2379-2382
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsAcesso restrito
dc.subjectHUMAN PARATHYROID HORMONEen
dc.subjectIMMUNOFLUOROMETRIC ASSAYen
dc.subjectHORMONE FRAGMENTSen
dc.subjectHYPERCALCEMIAen
dc.titleDEVELOPMENT AND CLINICAL-APPLICATION OF AN IMMUNOFLUOROMETRIC ASSAY FOR INTACT PARATHYROID-HORMONEen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationESCOLA PAULISTA MED,DEPT MED,CAIXA POSTAL 20266,BR-04034970 SAO PAULO,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT MED,CAIXA POSTAL 20266,BR-04034970 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.identifier.wosWOS:A1994PM04100007


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