DEVELOPMENT AND CLINICAL-APPLICATION OF AN IMMUNOFLUOROMETRIC ASSAY FOR INTACT PARATHYROID-HORMONE

DEVELOPMENT AND CLINICAL-APPLICATION OF AN IMMUNOFLUOROMETRIC ASSAY FOR INTACT PARATHYROID-HORMONE

Author Vieira, Jose Gilberto Henriques Autor UNIFESP Google Scholar
Nishida, Sonia Kiyomi Autor UNIFESP Google Scholar
Kasamatsu, Teresa Sayoko Autor UNIFESP Google Scholar
Amarante, E. C. Google Scholar
Kunii, Ilda Sizue Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Parathyroid hormone (PTH) is a linear peptide of 84 amino acids that is found in serum mainly in the form of carboxyl-terminal fragments. The biological activity of PTH depends on the presence of the amino-terminal portion and in circulation is limited to the intact molecule. We describe an immunofluorometric assay for the measurement of PTH-(1-84) based on a chicken egg yolk-derived amino-terminal antibody bound to microtiter plates by an anti-chicken Ig monoclonal antibody. As tracer antibody we employed a Europium-labelled carboxyl-terminal specific monoclonal antibody produced from a mouse immunized with hPTH-(53-84)-BSA conjugate. The assay included an initial overnight incubation of the sample and the solid phase-bound amino-terminal antibody, followed by washing and addition of the tracer antibody, and an additional two hours of incubation prior to fluorescence reading. The least-detectable dose was in the order of 2.5 pg/ml and preliminary studies in 40 normal adults showed values in the range of 4 to 70 pg/ml; for 12 patients with surgery-proven primary hyperparathyroidism values ranged from 109 to 743 pg/ml and for 34 patients with humoral hypercalcemia of malignancy from 2.5 to 66 pg/ml. We conclude that this assay, with its increased sensitivity and specificity, will be a valuable tool in the study of PTH secretion in normal and pathological situations.
Keywords HUMAN PARATHYROID HORMONE
IMMUNOFLUOROMETRIC ASSAY
HORMONE FRAGMENTS
HYPERCALCEMIA
Language English
Date 1994-10-01
Published in Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 10, p. 2379-2382, 1994.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Assoc Bras Divulg Cientifica
Extent 2379-2382
Access rights Closed access
Type Article
Web of Science ID WOS:A1994PM04100007
URI http://repositorio.unifesp.br/11600/43046

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