Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus

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2012-11-01
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Kashiwabuchi, Renata Tiemi [UNIFESP]
Khan, Yasin
Carvalho, Fabio Ramos de Souza [UNIFESP]
Hirai, Flávio Eduardo [UNIFESP]
Campos, Mauro Silveira de Queiroz [UNIFESP]
McDonnell, Peter John
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Purpose: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).Methods: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm(2), 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of viable but non-culturable microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition.Results: No difference was observed among the treatment group and the control samples (p=1).Conclusion: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.
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Arquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 75, n. 6, p. 423-426, 2012.
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