Show simple item record

dc.contributor.authorSantos, Leonardo dos [UNIFESP]
dc.contributor.authorMello, Ana.f.s. [UNIFESP]
dc.contributor.authorAntonio, Ednei Luiz [UNIFESP]
dc.contributor.authorTucci, Paulo José Ferreira [UNIFESP]
dc.date.accessioned2015-06-14T13:38:26Z
dc.date.available2015-06-14T13:38:26Z
dc.date.issued2008-03-01
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2008005000007
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 3, p. 199-201, 2008.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/4268
dc.description.abstractTriphenyltetrazolium chloride (TTC) staining and echocardiography (ECHO) are methods used to determine experimental myocardial infarction (MI) size, whose practical applicability should be expanded. Our objectives were to analyze the accuracy of ECHO in determining infarction size in rats during the first days following coronary occlusion and to test whether a simplified single measurement by TTC correctly indicates MI size, as determined by the average value for multiple slices. Infarction was induced in female Wistar rats by coronary artery occlusion and MI size analysis was performed after the acute (7th day) and chronic periods (after 4 weeks) by ECHO matched with TTC. ECHO and TTC showed similar values of MI size (% of left ventricle perimeter) in acute (ECHO: 33 ± 11, TTC: 35 ± 14) and chronic (ECHO: 38 ± 14, TTC: 39 ± 13 periods), and also presented an excellent correlation (r = 0.92, P < 0.001). Although measurements from different heart planes showed discrepancies, a single measurement acquired from the mid-ventricular level by TTC was a good estimate of MI size calculated by the average of multiple planes, with minimal disagreement (Bland-Altman test with mean ratio bias of 0.99 ± 0.07) and close to an ideal correlation (r = 0.99, P < 0.001). In the present study, ECHO was confirmed as a useful method for the determination of MI size even in the acute phase. Also, the single measure of a mid-ventricular section proposed as a simplification of the TTC method is a satisfactory prediction of average MI extension.en
dc.format.extent199-201
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectMyocardial infarction sizeen
dc.subjectEchocardiography and triphenyltetrazolium comparisonen
dc.subjectBland-Altman testen
dc.titleDetermination of myocardial infarction size in rats by echocardiography and tetrazolium staining: correlation, agreements, and simplificationsen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Medicina
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Fisiologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Medicina
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Fisiologia
dc.identifier.fileS0100-879X2008000300004.pdf
dc.identifier.scieloS0100-879X2008000300004
dc.identifier.doi10.1590/S0100-879X2008005000007
dc.description.sourceSciELO
dc.identifier.wosWOS:000253882200004


Files in this item

This item appears in the following Collection(s)

Show simple item record