Significance of AgNOR measurement in thyroid lesions
Camargo, Roberto Souza
Shirata, Neuza Kasumi
di Loreto, Celso
Garcia, Eduardo Anselmo
Longatto Filho, Adhernar
Is part ofAnalytical And Quantitative Cytology And Histology
MetadataShow full item record
OBJECTIVE: To evaluate the discriminating potential of AgNOR area measurement and count in thyroid tumors using static cytometry equipment.STUDY DESIGN: Slides were analyzed by a computerized system for image analysis, CAS 200 (Becton & Dickinson, U.S.A.), using the Cell Measurement computer program WAS 200, Becton & Dickinson). The argyrophilic reaction (NORs) was evaluated with a 400-fold amplification directly from the computer monitor.RESULTS: Thirty-three cases were analyzed for AgNOR staining. The cases studied included 3 goiters, 10 follicular adenomas, 6 Hurthle adenomas, 4 follicular carcinomas, 7 papillary carcinomas, and 3 Hurthle carcinomas. A total of 6,600 nuclei were evaluated. For statistical purposes, lesions were classified as benign and malignant, and both the number and the area of counted NORs showed very similar values. The NORs median among 19 benign tumors was 1.484 (SD +/- 0.265) and of 14 malignant tumors was 1.436 (SD +/- 0.414); the NORs areas were 2.6584 (SD +/- 1.0653) and 2.3643 (SD +/- 0.6320), respectively.CONCLUSION: Our results showed that AgNOR evaluation was not a significant parameter to discriminate between malignant and benign thyroid lesions.
CitationAnalytical And Quantitative Cytology And Histology. St Louis: Sci Printers & Publ Inc, v. 28, n. 4, p. 188-192, 2006.
- EPM - Artigos