Safety and elicitation of humoral and cellular responses in Colombian malaria-naive volunteers by a Plasmodium vivax circumsporozoite protein-derived synthetic vaccine
Perlaza, Blanca Liliana
Fernandez, Olga Lucia
Lenis, Ana Milena
Acuna, Lina Maria
Velez, Juan Diego
Palacios, Ricardo [UNIFESP]
Is part ofAmerican Journal Of Tropical Medicine And Hygiene
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Substantial experimental evidence indicates that the Plasmodium circurnsporozoite (CS) protein has great potential as a vaccine candidate. We tested the safety and immunogenicity of vaccines composed of P. vivax CS-derived synthetic peptides. Sixty-nine healthy, malaria-naive volunteers were randomized to receive three injections of placebo or synthetic proteins N, R, or C (10 30, or 100 mu g/dose) in a double-blinded fashion. Vaccines were well tolerated and no serious adverse events were observed. Peptides N and R elicited humoral responses at all doses; peptide C elicicted these responses only at doses of 30 and 100 mu g. The N peptide at a dose of 100 mu g elicited the greatest antibody response. Antibodies to the three peptides recognized P. vivax sporozoites in an immunofluorescent antibody test. Peripheral blood mononuclear cells from most immunized volunteers also produced interferon-gamma upon peptide in vitro stimulation. These vaccines appear safe, well tolerated, and immunogenic in malaria-naive volunteers. Further optimization and development of this vaccine is being attempted to conduct phase 11 clinical trials.
CitationAmerican Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 73, n. 5, p. 3-9, 2005.
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